Search: for Help
Victors Home
Introduction
Statistics
News and Updates
Victors Query
Victors BLAST
Data Submission
Data Download
OHPI SPARQL Query
Documentation
FAQs
Disclaimer
Contact Us
UMMS Logo

Victors Search

Found 570 gene(s). Export as Excel file, text file

<
Victors ID Gene Name Sequence Strain (Species/Organism) NCBI Gene ID NCBI Nucleotide GI NCBI Protein GI Locus Tag Genbank Accession Protein Accession Protein Name Molecule Role Molecule Role Annotation PMID
2 traT from Escherichia coli (strain: A2363) Escherichia coli (strain: A2363) 3244810 58000389 O2R_18 YP_190117.1 plays a role in APEC complement resistance; similar to protein family HMM PF05818 Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics (Sukupolvi and O'Connor, 1987). 3323837
3 traT from Escherichia coli EH41 Escherichia coli EH41 3654467 73853290 LH0131 YP_308786.1 Complement resistance protein Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics (Sukupolvi and O'Connor, 1987). 3323837
4 ehxA Escherichia coli 3654480 73853298 LH0144 AY258503 YP_308794 hemolysin A Virulence factor TOXIN: Ehx demonstrated lysis of BL-3 cells (cells from a bovine lymphoma cell line), sheep and human erythrocytes (Bauer and Welch, 1996). 8557336
5 ehxC Escherichia coli 3654481 73853299 LH0145 AY258503 YP_308795 hemolysin C Virulence factor TOXIN: Ehx demonstrated lysis of BL-3 cells (cells from a bovine lymphoma cell line), sheep and human erythrocytes (Bauer and Welch, 1996). 8557336
6 eatA from Escherichia coli (strain: JEF100) Escherichia coli (strain: JEF100) 3829299 83404842 pCoo056 YP_424856.1 secreted autotransporter serine protease EatA Virulence factor MUTATION: interruption of the eatA gene retarded fluid accumulation in the rabbit ileal loop model, suggesting that this autotransporter contributes to the virulence of ETEC (Patel et al., 2004). 14977988
7 traT from Escherichia coli (strain: A2363) Escherichia coli (strain: A2363) 3853397 84060756 O2ColV112 YP_443958.1 surface exclusion protein; similar to GenBank Accession Number AAW58918 Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics (Sukupolvi and O'Connor, 1987). 3323837
8 iroB from Escherichia coli (strain: A2363) Escherichia coli (strain: A2363) 3853505 84060896 O2ColV53 YP_444106.1 iron-related glycotransferase; similar to NP_753168 Virulence factor MUTATION: attenuated in chicks (Caza et al., 2008). 18541653
9 iroC from Escherichia coli (strain: A2363) Escherichia coli (strain: A2363) 3853506 84060897 O2ColV54 YP_444107.1 iron-related ABC transporter protein; similar to GenBank Accession Number AAN76099 Virulence factor MUTATION: attenuated in chicks (Caza et al., 2008). 18541653
10 iroD from Escherichia coli (strain: A2363) Escherichia coli (strain: A2363) 3853507 84060898 O2ColV55 YP_444108.1 iron-related ferric enterochelin esterase; similar to GenBank Accession Number AAN76100 Virulence factor MUTATION: attenuated in chicks (Caza et al., 2008). 18541653
11 iroN from Escherichia coli (strain: A2363) Escherichia coli (strain: A2363) 3853509 84060900 O2ColV57 YP_444110.1 iron-related siderophore receptor; similar to GenBank Accession Number AAN76093 Virulence factor MUTATION: iroA gene cluster mutants were unable to resist Lcn2 in mouse serum (Fischbach et al., 2006). 17060628
12 iucA from Escherichia coli (strain: A2363) Escherichia coli (strain: A2363) 3853516 84060856 O2ColV11 YP_444058.1 aerobactin biosynthesis protein; similar to GenBank Accession Number CAA53707 Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
13 iucB from Escherichia coli (strain: A2363) Escherichia coli (strain: A2363) 3853517 84060857 O2ColV12 YP_444059.1 N-hydroxylysine acetylase; aerobactin biosynthesis protein; similar to GenBank Accession Number CAH64815 Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
14 iucC from Escherichia coli (strain: A2363) Escherichia coli (strain: A2363) 3853518 84060858 O2ColV13 YP_444060.1 aerobactin biosynthesis protein; similar to GenBank Accession Number CAH64814 Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
15 iucD from Escherichia coli (strain: A2363) Escherichia coli (strain: A2363) 3853519 84060859 O2ColV14 YP_444061.1 L-lysine 6-monooxigenase; similar to GenBank Accession Number CAE55773 Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
16 iutA from Escherichia coli (strain: A2363) Escherichia coli (strain: A2363) 3853520 84060860 O2ColV15 YP_444062.1 ferric aerobactin receptor; similar to GenBank Accession Number CAE55774 Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
17 traT from Escherichia coli (strain: DIJ1, serotype: O86:H-) Escherichia coli (strain: DIJ1, serotype: O86:H-) 4364265 116006905 pO86A1_p122 YP_788088.1 TraT complement resistance protein precursor Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics (Sukupolvi and O'Connor, 1987). 3323837
18 bfpA from Escherichia coli (strain: E2348/69, serotype: O127:H6) Escherichia coli (strain: E2348/69, serotype: O127:H6) 6382168 190014867 MAR003 YP_001965379.1 Major pilin structural unit bundlin Virulence factor MUTATION: bfpA deletion mutant of EPEC was less virulent in humans than the wild-type EPEC (Bieber et al., 1998). 9641917
19 bfpB from Escherichia coli (strain: E2348/69, serotype: O127:H6) Escherichia coli (strain: E2348/69, serotype: O127:H6) 6382170 190014869 MAR005 YP_001965381.1 Lipoprotein Virulence factor MUTATION: bfpB mutant EPEC is unable to form BFP which is a major virulence factor of EPEC (Ramer et al., 1996). 8932312
20 bfpE from Escherichia coli (strain: E2348/69, serotype: O127:H6) Escherichia coli (strain: E2348/69, serotype: O127:H6) 6382174 190014873 MAR009 YP_001965385.1 Transmembrane protein Virulence factor MUTATION: bfpE mutant strain of EPEC fails to form detectable BFP and fails to carry out autoaggregation and localized adherence to HEp-2 epithelial cells in tissue culture (Blank and Donnenberg, 2001). 11443077
21 bfpF from Escherichia coli (strain: E2348/69, serotype: O127:H6) Escherichia coli (strain: E2348/69, serotype: O127:H6) 6382175 190014874 MAR010 YP_001965386.1 Nucleotide binding protein Virulence factor MUTATION: bfpF mutant colonized the human intestine but was about 200-fold less virulent than the wild-type EPEC (Bieber et al., 1998). 9641917
22 bfpP from Escherichia coli (strain: E2348/69, serotype: O127:H6) Escherichia coli (strain: E2348/69, serotype: O127:H6) 6382176 190014875 MAR011 YP_001965387.1 Prepilin peptidase Virulence factor MUTATION: mutation in bfpP blocks BFP biogenesis and localized adherence to host cells by EPEC (Anantha et al., 2000). 10762251
23 perA Escherichia coli 6382186 190014885 MAR020 YP_001965397.1 Transcriptional activator, bfp operon Virulence factor MUTATION: bfpT (perA) disruption mutant of EPEC was less virulent in humans than the wild-type EPEC (Bieber et al., 1998). 9641917
2026 bfpA from Escherichia coli (strain: B171) Escherichia coli (strain: B171) 1238603 10955353 pB171_003 NP_053065.1 Bundlin Virulence factor MUTATION: bfpA deletion mutant of EPEC was less virulent in humans than the wild-type EPEC (Bieber et al., 1998). 9641917
2027 bfpB from Escherichia coli (strain: B171) Escherichia coli (strain: B171) 1238604 10955355 pB171_005 NP_053067.1 BfpB Virulence factor MUTATION: bfpB mutant EPEC is unable to form BFP which is a major virulence factor of EPEC (Ramer et al., 1996). 8932312
2028 bfpE from Escherichia coli (strain: B171) Escherichia coli (strain: B171) 1238607 10955359 pB171_009 NP_053071.1 BfpE Virulence factor MUTATION: bfpE mutant strain of EPEC fails to form detectable BFP and fails to carry out autoaggregation and localized adherence to HEp-2 epithelial cells in tissue culture (Blank and Donnenberg, 2001). 11443077
2029 bfpF from Escherichia coli (strain: B171) Escherichia coli (strain: B171) 1238608 10955360 pB171_010 NP_053072.1 BfpF Virulence factor MUTATION: bfpF mutant colonized the human intestine but was about 200-fold less virulent than the wild-type EPEC (Bieber et al., 1998). 9641917
2030 bfpP from Escherichia coli (strain: B171) Escherichia coli (strain: B171) 1238616 10955361 pB171_011 NP_053073.1 prepilin peptidase Virulence factor MUTATION: mutation in bfpP blocks BFP biogenesis and localized adherence to host cells by EPEC (Anantha et al., 2000). 10762251
2031 bfpT Escherichia coli 1238617 10955374 pB171_024 EF011039 NP_053086 BfpT Virulence factor MUTATION: bfpT (perA) disruption mutant of EPEC was less virulent in humans than the wild-type EPEC (Bieber et al., 1998). 9641917
2032 rfaH from Escherichia coli E24377A Escherichia coli E24377A 5586647 157157494 EcE24377A_4360 YP_001465326.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice (Nagy et al., 2002). 12117951
2033 dppA from Escherichia coli E24377A Escherichia coli E24377A 5586739 157159059 EcE24377A_4037 YP_001465018.1 dipeptide ABC transporter, periplasmic dipeptide-binding protein Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice (Haugen et al., 2007). 17074858
2034 fliC from Escherichia coli E24377A Escherichia coli E24377A 5586916 157159035 EcE24377A_2158 YP_001463228.1 flagellin FliC Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains (Erdem et al., 2007). 17693516
2035 ompA from Escherichia coli E24377A Escherichia coli E24377A 5589214 157159269 EcE24377A_1071 YP_001462189.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells (Prasadarao et al., 1999). 10531228
2036 fimH from Escherichia coli E24377A Escherichia coli E24377A 5589412 157157381 EcE24377A_4917 YP_001465845.1 protein FimH Virulence factor MUTATION: attenuated in mice and humans (Wiles et al., 2008). 18482721
2037 dsbA from Escherichia coli E24377A Escherichia coli E24377A 5590815 157155214 EcE24377A_4378 YP_001465340.1 thiol:disulfide interchange protein DsbA Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC (Donnenberg et al., 1997). 9224871
2038 dsbA from Escherichia coli HS Escherichia coli HS 5591486 157163329 EcHS_A4083 YP_001460647.1 thiol:disulfide interchange protein DsbA Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC (Donnenberg et al., 1997). 9224871
2039 fimH from Escherichia coli HS Escherichia coli HS 5591791 157163764 EcHS_A4546 YP_001461082.1 protein FimH Virulence factor MUTATION: attenuated in mice and humans (Wiles et al., 2008). 18482721
2040 ompA from Escherichia coli HS Escherichia coli HS 5591810 157160478 EcHS_A1066 YP_001457796.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells (Prasadarao et al., 1999). 10531228
2041 tonB from Escherichia coli HS Escherichia coli HS 5593249 157160757 EcHS_A1361 YP_001458075.1 outer membrane receptor-mediated transport energizer protein TonB Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
2042 dppA from Escherichia coli HS Escherichia coli HS 5593889 157163019 EcHS_A3744 YP_001460337.1 dipeptide ABC transporter, periplasmic dipeptide-binding protein Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice (Haugen et al., 2007). 17074858
2043 rfaH from Escherichia coli HS Escherichia coli HS 5594372 157163316 EcHS_A4064 YP_001460634.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice (Nagy et al., 2002). 12117951
2044 mppA Escherichia coli APEC O1 4491551 117623583 APECO1_483 CP000468 YP_852496 periplasmic murein peptide-binding protein precursor Virulence factor MUTATION: mppA mutant is attenuated in chickens (Li et al., 2005). 15845486
2045 sbmA Escherichia coli APEC O1 4491947 117622640 APECO1_1631 CP000468 YP_851553 transport protein Virulence factor MUTATION: sbmA mutant is attenuated in chickens (Li et al., 2005). 15845486
2046 fimH from Escherichia coli APEC O1 Escherichia coli APEC O1 4492146 117626628 APECO1_2110 YP_859951.1 type 1 fimbiral adhesin FimH Virulence factor MUTATION: attenuated in mice and humans (Wiles et al., 2008). 18482721
2047 ychF Escherichia coli 4492443 117623421 APECO1_319 YP_852334.1 translation-associated GTPase Virulence factor MUTATION: ychF mutant is attenuated in chickens (Li et al., 2005). 15845486
2048 waaL Escherichia coli APEC O1 4492586 117625899 APECO1_2834 CP000468 YP_859222 lipid A-core, surface polymer ligase Virulence factor MUTATION: waaL mutant is attenuated in chickens (Li et al., 2005). 15845486
2049 dppA from Escherichia coli APEC O1 Escherichia coli APEC O1 4492758 117625828 APECO1_2904 YP_859151.1 dipeptide transporter protein DppA Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice (Haugen et al., 2007). 17074858
2050 dsbA from Escherichia coli APEC O1 Escherichia coli APEC O1 4492849 117626134 APECO1_2601 YP_859457.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC (Donnenberg et al., 1997). 9224871
2051 ibeA from Escherichia coli APEC O1 Escherichia coli APEC O1 4493341 117626645 APECO1_2093 YP_859968.1 invasion protein IbeA Virulence factor MUTATION: deletion mutant was significantly less invasive in human brain microvascular endothelial cells (BMECs) than the parent strain (E. coli K1) (Huang et al., 2001). 11237832
2052 papA from Escherichia coli APEC O1 Escherichia coli APEC O1 4493551 117625209 APECO1_3519 YP_854256.1 major fibrial protein Virulence factor MUTATION: mutant papA in wild-type smooth strains do not mediate Gal-Gal adherence (Johnson, 1991). 1672263
2053 sitB Escherichia coli 4493636 117623375 APECO1_273 YP_852288.1 ABC Mn+2/Fe+2 transporter, ATPase subunit SitB Virulence factor MUTATION: sitB mutant is attenuated in chickens (Li et al., 2005). 15845486
2054 malX Escherichia coli APEC O1 4493654 117623807 APECO1_704 CP000468 YP_852720 bifunctional PTS system maltose and glucose-specific transporter subunits IICB Virulence factor MUTATION: malX mutant is attenuated in chickens (Li et al., 2005). 15845486
2055 ompA from Escherichia coli APEC O1 Escherichia coli APEC O1 4493784 117623167 APECO1_62 YP_852080.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells (Prasadarao et al., 1999). 10531228
2056 tia from Escherichia coli APEC O1 Escherichia coli APEC O1 4493822 117625198 APECO1_3530 YP_854245.1 Tia invasion determinant Virulence factor MUTATION: tia deletion strains were noninvasive and lacked the ability to adhere to human ileocecal cells (Fleckenstein et al., 1996). 8675335
2057 papG from Escherichia coli APEC O1 Escherichia coli APEC O1 4493827 117625201 APECO1_3527 YP_854248.1 allele II Virulence factor MUTATION: attenuated to human neutrophils in vitro (Tewari et al., 1994). 7960108
2058 rfaH from Escherichia coli APEC O1 Escherichia coli APEC O1 4494179 117626120 APECO1_2615 YP_859443.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice (Nagy et al., 2002). 12117951
2059 kpsS Escherichia coli APEC O1 4494206 117625251 APECO1_3477 CP000468 YP_854344 polysialic acid capsule synthesis protein KpsS Virulence factor MUTATION: kpsS mutant is attenuated in chickens (Li et al., 2005). 15845486
2060 wcaE Escherichia coli APEC O1 4494493 117624251 APECO1_1145 CP000468 YP_853164 putative glycosyl transferase Virulence factor MUTATION: wcaE mutant is attenuated in chickens (Li et al., 2005). 15845486
2061 APECO1_3367 Escherichia coli APEC O1 4494532 117625360 APECO1_3367 CP000468 YP_855140 putative Yqi fimbrial adhesin Virulence factor MUTATION: yqi mutant is attenuated in chickens (Antão et al., 2009). 19907658
2062 fliC from Escherichia coli APEC O1 Escherichia coli APEC O1 4494642 117624065 APECO1_964 YP_852978.1 flagellin Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains (Erdem et al., 2007). 17693516
2063 yjjQ Escherichia coli APEC O1 4494948 117626679 APECO1_2059 CP000468 YP_860002 hypothetical protein Virulence factor MUTATION: yjjQ mutant is attenuated in chickens (Li et al., 2008). 18375801
2064 chuA from Escherichia coli APEC O1 Escherichia coli APEC O1 4495129 117625783 APECO1_2948 YP_859106.1 outer membrane heme/hemoglobin receptor ChuA Virulence factor MUTATION: chuA mutant unable to grow on iron-depleted medium containing heme (Torres and Payne, 1997). 9157252
2065 tktA Escherichia coli APEC O1 4495170 117625164 APECO1_3594 CP000468 YP_854152 transketolase Virulence factor MUTATION: tktA mutant is attenuated in chickens (Li et al., 2005). 15845486
2066 tonB from Escherichia coli APEC O1 Escherichia coli APEC O1 4495352 117623468 APECO1_368 YP_852381.1 membrane spanning protein in TonB-ExbB-ExbD complex; transduces proton motive force of the cytoplasmic membrane to outer membrane transporters; involved in the transport of ron-siderophore complexes, vitamin B12 and colicins Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
2067 rfaH from Escherichia coli 536 Escherichia coli 536 4187921 110644186 ECP_4055 YP_671916.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice (Nagy et al., 2002). 12117951
2068 ECP_4655 Escherichia coli 536 4190232 110644762 ECP_4655 CP000247 YP_672492 FimH protein precursor Virulence factor MUTATION: attenuated in mice and humans (Wiles et al., 2008). 18482721
2069 dppA from Escherichia coli UTI89 Escherichia coli UTI89 3989640 91213056 UTI89_C4082 YP_543042.1 periplasmic dipeptide transport protein precursor Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice (Haugen et al., 2007). 17074858
2070 cnf1 Escherichia coli UTI89 3990770 91213869 UTI89_C4921 CP000243 YP_543855 cytotoxic necrotizing factor 1 Virulence factor MUTATION: attenuated in mice (Rippere-Lampe et al., 2001). 11349064
2071 hlyA from Escherichia coli UTI89 Escherichia coli UTI89 3990775 91213874 UTI89_C4926 YP_543860.1 hemolysin A Virulence factor MUTATION: attenuated in zebra fish (Wiles et al., 2009). 20019794
2072 hlyC from Escherichia coli UTI89 Escherichia coli UTI89 3990776 91213875 UTI89_C4927 YP_543861.1 hemolysin C Virulence factor MUTATION: hlyC is required for the acylation and therefore activation of the toxin hemolysin which has the ability to lyse several different types of cells (Stanley et al., 1996). 8793877
2073 chuA from Escherichia coli UTI89 Escherichia coli UTI89 3991259 91213002 UTI89_C4028 YP_542988.1 outer membrane hemin receptor ChuA Virulence factor MUTATION: chuA mutant unable to grow on iron-depleted medium containing heme (Torres and Payne, 1997). 9157252
2074 iroN from Escherichia coli UTI89 Escherichia coli UTI89 3991364 91210147 UTI89_C1118 YP_540133.1 IroN protein Virulence factor MUTATION: iroA gene cluster mutants were unable to resist Lcn2 in mouse serum (Fischbach et al., 2006). 17060628
2075 iroD from Escherichia coli UTI89 Escherichia coli UTI89 3991366 91210149 UTI89_C1120 YP_540135.1 IroD protein Virulence factor MUTATION: attenuated in chicks (Caza et al., 2008). 18541653
2076 iroC from Escherichia coli UTI89 Escherichia coli UTI89 3991367 91210150 UTI89_C1121 YP_540136.1 IroC protein Virulence factor MUTATION: attenuated in chicks (Caza et al., 2008). 18541653
2077 iroB from Escherichia coli UTI89 Escherichia coli UTI89 3991368 91210151 UTI89_C1122 YP_540137.1 putative glucosyltransferase Virulence factor MUTATION: attenuated in chicks (Caza et al., 2008). 18541653
2078 tonB from Escherichia coli UTI89 Escherichia coli UTI89 3991369 91210476 UTI89_C1451 YP_540462.1 membrane spanning protein in TonB-ExbB-ExbD complex; transduces proton motive force of the cytoplasmic membrane to outer membrane transporters; involved in the transport of ron-siderophore complexes, vitamin B12 and colicins Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
2079 papG from Escherichia coli UTI89 Escherichia coli UTI89 3992036 91213835 UTI89_C4887 YP_543821.1 p pilus adhesin PapG protein Virulence factor MUTATION: attenuated to human neutrophils in vitro (Tewari et al., 1994). 7960108
2080 papA from Escherichia coli UTI89 Escherichia coli UTI89 3992044 91213843 UTI89_C4895 YP_543829.1 major pilin subunit PapA Virulence factor MUTATION: mutant papA in wild-type smooth strains do not mediate Gal-Gal adherence (Johnson, 1991). 1672263
2081 fimH from Escherichia coli UTI89 Escherichia coli UTI89 3992235 91213965 UTI89_C5017 YP_543951.1 type 1 fimbiral adhesin FimH Virulence factor MUTATION: attenuated in mice and humans (Wiles et al., 2008). 18482721
2082 ibeA from Escherichia coli UTI89 Escherichia coli UTI89 3992253 91213983 UTI89_C5035 YP_543969.1 invasion protein IbeA Virulence factor MUTATION: deletion mutant was significantly less invasive in human brain microvascular endothelial cells (BMECs) than the parent strain (E. coli K1) (Huang et al., 2001). 11237832
2083 aslA from Escherichia coli UTI89 Escherichia coli UTI89 3992515 91213321 UTI89_C4359 YP_543307.1 arylsulfatase Virulence factor MUTATION: disruption mutant was less invasive in an in vitro HBMEC invasion assay than the wild-type E. coli K1 (Hoffman et al., 2000). 10948126
2084 dsbA from Escherichia coli UTI89 Escherichia coli UTI89 3992605 91213404 UTI89_C4447 YP_543390.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC (Donnenberg et al., 1997). 9224871
2085 fliC from Escherichia coli UTI89 Escherichia coli UTI89 3993212 91211142 UTI89_C2124 YP_541128.1 flagellin Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains (Erdem et al., 2007). 17693516
2086 rfaH from Escherichia coli UTI89 Escherichia coli UTI89 3993833 91213389 UTI89_C4427 YP_543375.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice (Nagy et al., 2002). 12117951
2087 ompA from Escherichia coli UTI89 Escherichia coli UTI89 3994721 91210052 UTI89_C1022 YP_540038.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells (Prasadarao et al., 1999). 10531228
2088 iroN from Escherichia coli CFT073 Escherichia coli CFT073 1034931 26247124 c1250 NP_753164.1 Siderophore receptor IroN Virulence factor MUTATION: iroA gene cluster mutants were unable to resist Lcn2 in mouse serum (Fischbach et al., 2006). 17060628
2089 iroD from Escherichia coli CFT073 Escherichia coli CFT073 1034933 26247126 c1252 NP_753166.1 Ferric enterochelin esterase Virulence factor MUTATION: attenuated in chicks (Caza et al., 2008). 18541653
2090 iroC from Escherichia coli CFT073 Escherichia coli CFT073 1034934 26247127 c1253 NP_753167.1 ATP binding cassette (ABC) transporter homolog Virulence factor MUTATION: attenuated in chicks (Caza et al., 2008). 18541653
2091 iroB from Escherichia coli CFT073 Escherichia coli CFT073 1034935 26247128 c1254 NP_753168.1 Putative glucosyltransferase Virulence factor MUTATION: attenuated in chicks (Caza et al., 2008). 18541653
2092 tonB from Escherichia coli CFT073 Escherichia coli CFT073 1035499 26247582 c1717 NP_753622.1 membrane spanning protein in TonB-ExbB-ExbD complex; transduces proton motive force of the cytoplasmic membrane to outer membrane transporters; involved in the transport of ron-siderophore complexes, vitamin B12 and colicins Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
2093 ycjM Escherichia coli CFT073 1035566 26247644 c1780 AE014075 NP_753684 putative sucrose phosphorylase Virulence factor MUTATION: ycjM mutant is attenuated in chickens (Li et al., 2005). 15845486
2094 rfaH from Escherichia coli CFT073 Escherichia coli CFT073 1035846 26250608 c4789 NP_756648.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice (Nagy et al., 2002). 12117951
2095 wecE Escherichia coli CFT073 1036493 26250530 c4711 AE014075 NP_756570 TDP-4-oxo-6-deoxy-D-glucose transaminase Virulence factor MUTATION: wecE mutant is attenuated in mice (Bahrani-Mougeot et al., 2002). 12180926
2096 dsbA from Escherichia coli CFT073 Escherichia coli CFT073 1036525 26250621 c4804 NP_756661.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC (Donnenberg et al., 1997). 9224871
2097 focA Escherichia coli CFT073 1036891 26247113 c1239 AE014075 NP_753153 F1C major fimbrial subunit precursor Virulence factor MUTATION: focA deletion mutants were unable to bind to brain glycolipids (Prasadarao et al., 1993). 8098039
2098 fliC from Escherichia coli CFT073 Escherichia coli CFT073 1036977 26248190 c2338 NP_754230.1 flagellin Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains (Erdem et al., 2007). 17693516
2099 chuA from Escherichia coli CFT073 Escherichia coli CFT073 1037196 26250130 c4308 NP_756170.1 Outer membrane heme/hemoglobin receptor Virulence factor MUTATION: chuA mutant unable to grow on iron-depleted medium containing heme (Torres and Payne, 1997). 9157252
2100 fimH from Escherichia coli CFT073 Escherichia coli CFT073 1037233 26251208 c5400 NP_757248.1 FimH protein precursor Virulence factor MUTATION: attenuated in mice and humans (Wiles et al., 2008). 18482721
2101 fimF Escherichia coli CFT073 1037237 26251205 c5397 AE014075 NP_757245 FimF protein precursor Virulence factor MUTATION: fimF mutant is attenuated in mice (Bahrani-Mougeot et al., 2002). 12180926
2102 fimD Escherichia coli CFT073 1037242 26251204 c5396 AE014075 NP_757244 Outer membrane usher protein fimD precursor Virulence factor MUTATION: fimD mutant is attenuated in mice (Bahrani-Mougeot et al., 2002). 12180926
2103 fimC Escherichia coli CFT073 1037243 26251203 c5395 AE014075 NP_757243 chaperone protein fimC precursor Virulence factor MUTATION: fimC mutant is attenuated in mice (Bahrani-Mougeot et al., 2002). 12180926
2104 fimI from E. coli CFT073 Escherichia coli CFT073 1037245 26251202 c5394 NP_757242.1 Fimbrin-like protein fimI precursor Virulence factor MUTATION: fimI mutant is attenuated in mice (Bahrani-Mougeot et al., 2002). 12180926
2105 fimB Escherichia coli CFT073 1037250 26251199 c5391 AE014075 NP_757239 tyrosine recombinase Virulence factor MUTATION: fimB mutant is attenuated in mice (Bahrani-Mougeot et al., 2002). 12180926
2106 dppA from Escherichia coli CFT073 Escherichia coli CFT073 1037335 26250183 c4361 NP_756223.1 Periplasmic dipeptide transport protein precursor Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice (Haugen et al., 2007). 17074858
2107 manB from Escherichia coli CFT073 Escherichia coli CFT073 1037835 26248405 c2557 NP_754445.1 Phosphomannomutase Virulence factor MUTATION: manB mutant is attenuated in mice (Bahrani-Mougeot et al., 2002). 12180926
2108 waaW Escherichia coli CFT073 1038245 26250272 c4450 AE014075 NP_756312 UDP-galactose:(galactosyl) LPS alpha1,2-galactosyltransferase Virulence factor MUTATION: waaW mutant is attenuated in chickens (Li et al., 2005). 15845486
2109 hlyC from Escherichia coli CFT073 Escherichia coli CFT073 1039495 26249404 c3569 NP_755444.1 Hemolysin C Virulence factor MUTATION: hlyC is required for the acylation and therefore activation of the toxin hemolysin which has the ability to lyse several different types of cells (Stanley et al., 1996). 8793877
2110 hlyA from Escherichia coli CFT073 Escherichia coli CFT073 1039496 26249405 c3570 NP_755445.1 Hemolysin A Virulence factor MUTATION: attenuated in zebra fish (Wiles et al., 2009). 20019794
2111 papG from Escherichia coli CFT073 Escherichia coli CFT073 1039518 26249418 c3583 NP_755458.1 PapG protein Virulence factor MUTATION: attenuated to human neutrophils in vitro (Tewari et al., 1994). 7960108
2112 papA from Escherichia coli CFT073 Escherichia coli CFT073 1039530 26249427 c3592 NP_755467.1 PapA protein Virulence factor MUTATION: mutant papA in wild-type smooth strains do not mediate Gal-Gal adherence (Johnson, 1991). 1672263
2113 sat from Escherichia coli CFT073 Escherichia coli CFT073 1039573 26249454 c3619 NP_755494.1 Aecreted auto transpoter toxin Virulence factor MUTATION: Sat elicits cytopathic activity that damages host tissue and may increase the ability of E. coli to propagate (Guyer et al., 2002). 12117966
2114 iutA from Escherichia coli CFT073 Escherichia coli CFT073 1039578 26249458 c3623 NP_755498.1 IutA protein Virulence factor MUTATION: mutant attenuated in mice competition assay (Torres et al., 2001). 11553558
2115 iucD from Escherichia coli CFT073 Escherichia coli CFT073 1039579 26249459 c3624 NP_755499.1 IucD protein Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
2116 iucC from Escherichia coli CFT073 Escherichia coli CFT073 1039580 26249460 c3625 NP_755500.1 IucC protein Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
2117 iucB from Escherichia coli CFT073 Escherichia coli CFT073 1039581 26249461 c3626 NP_755501.1 IucB protein Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
2118 iucA from Escherichia coli CFT073 Escherichia coli CFT073 1039582 26249462 c3627 NP_755502.1 IucA protein Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
2119 kpsC Escherichia coli CFT073 1039730 26249525 c3690 AE014075 NP_755565 KpsC protein Virulence factor MUTATION: kpsC mutant is attenuated in mice (Bahrani-Mougeot et al., 2002). 12180926
2120 pyrD Escherichia coli CFT073 1039998 26246966 c1081 AE014075 NP_753006 dihydroorotate dehydrogenase 2 Virulence factor MUTATION: pyrD mutant is attenuated in mice (Bahrani-Mougeot et al., 2002). 12180926
2121 ompA from Escherichia coli CFT073 Escherichia coli CFT073 1039999 26246978 c1093 NP_753018.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells (Prasadarao et al., 1999). 10531228
2122 phoU Escherichia coli CFT073 1040241 161486057 c4648 AE014075 NP_756506 transcriptional regulator PhoU Virulence factor MUTATION: phoU mutant is attenuated in mice (Bahrani-Mougeot et al., 2002). 12180926
2123 rfaH from Escherichia coli O157:H7 str. Sakai Escherichia coli O157:H7 str. Sakai 915134 15834024 ECs4770 NP_312797.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice (Nagy et al., 2002). 12117951
2124 stx1B Escherichia coli O157:H7 str. EDL933 956702 15802645 Z3343 AE005174 NP_288672 shiga-like toxin 1 subunit B encoded within prophage CP-933V Virulence factor TOXIN: Stx induces apoptosis in HeLa cells (Cherla et al., 2003). 14638419
2125 map from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 956892 15799850 Z0178 NP_285862.1 methionine aminopeptidase Virulence factor MUTATION: map mutant is attenuated in calves (Dziva et al., 2004). 15528651
2126 Z3341 Escherichia coli O157:H7 str. EDL933 956966 15802643 Z3341 AE005174 NP_288670 hypothetical protein Virulence factor MUTATION: Z3341 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2127 stx1A Escherichia coli O157:H7 str. EDL933 956968 15802646 Z3344 AE005174 NP_288673 shiga-like toxin 1 subunit A encoded within prophage CP-933V Virulence factor TOXIN: Stx induces apoptosis in HeLa cells (Cherla et al., 2003). 14638419
2128 Z0275 Escherichia coli O157:H7 str. EDL933 957027 15799923 Z0275 AE005174 NP_285935 hypothetical protein Virulence factor MUTATION: Z0275 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2129 sanA Escherichia coli O157:H7 str. EDL933 957084 15802700 Z3399 AE005174 NP_288727 hypothetical protein Virulence factor MUTATION: sanA mutant is attenuated in calves (Dziva et al., 2004). 15528651
2130 mglA Escherichia coli O157:H7 str. EDL933 957113 15802705 Z3404 AE005174 NP_288732 galactose/methyl galaxtoside transporter ATP-binding protein Virulence factor MUTATION: mglA mutant is attenuated in calves (van et al., 2005). 15731074
2131 Z0390 Escherichia coli O157:H7 str. EDL933 957204 15800026 Z0390 AE005174 NP_286038 hypothetical protein Virulence factor MUTATION: Z0390 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2132 Z3496 Escherichia coli O157:H7 str. EDL933 957222 15802787 Z3496 AE005174 NP_288814 hypothetical protein Virulence factor MUTATION: Z3496 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2133 Z0469 Escherichia coli 957315 15800100 Z0469 NP_286112.1 putative structural protein (partial) Virulence factor MUTATION: Z0469 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2134 rdgC Escherichia coli O157:H7 str. EDL933 957342 15800120 Z0492 AE005174 NP_286132 recombination associated protein Virulence factor MUTATION: rdgC mutant is attenuated in calves (Dziva et al., 2004). 15528651
2135 Z0609 Escherichia coli O157:H7 str. EDL933 957466 15800217 Z0609 AE005174 NP_286229 hypothetical protein Virulence factor MUTATION: Z0609 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2136 evgS Escherichia coli O157:H7 str. EDL933 957555 15802913 Z3632 AE005174 NP_288940 hybrid sensory histidine kinase in two-component regulatory system with EvgA Virulence factor MUTATION: evgS mutant is attenuated in calves (van et al., 2005). 15731074
2137 yfhH Escherichia coli 957907 161367545 Z3841 NP_289117.2 putative DNA-binding transcriptional regulator Virulence factor MUTATION: yfhH mutant is attenuated in calves (Dziva et al., 2004). 15528651
2138 trxC Escherichia coli O157:H7 str. EDL933 957981 15803110 Z3867 AE005174 NP_289141 thioredoxin 2 Virulence factor MUTATION: trxC mutant is attenuated in calves (Dziva et al., 2004). 15528651
2139 Z0990 Escherichia coli O157:H7 str. EDL933 957986 15800523 Z0990 AE005174 NP_286535 hypothetical protein Virulence factor MUTATION: Z0990 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2140 Z3950 Escherichia coli O157:H7 str. EDL933 958085 15803172 Z3950 AE005174 NP_289204 hypothetical protein Virulence factor MUTATION: Z3950 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2141 recD Escherichia coli O157:H7 str. EDL933 958288 15803338 Z4136 AE005174 NP_289371 exonuclease V subunit alpha Virulence factor MUTATION: recD mutant is attenuated in calves (van et al., 2005). 15731074
2142 kduI Escherichia coli O157:H7 str. EDL933 958313 15803363 Z4163 AE005174 NP_289396 5-keto-4-deoxyuronate isomerase Virulence factor MUTATION: kduI mutant is attenuated in calves (Dziva et al., 2004). 15528651
2143 Z4200 Escherichia coli O157:H7 str. EDL933 958343 15803400 Z4200 AE005174 NP_289433 hypothetical protein Virulence factor MUTATION: z4200 (pkgA) mutant is attenuated in calves (van et al., 2005). 15731074
2144 Z3635 Escherichia coli O157:H7 str. EDL933 958378 15802914 Z3635 AE005174 NP_288942 putative transporter YfdV Virulence factor MUTATION: z3635 (yfdV) mutant is attenuated in calves (van et al., 2005). 15731074
2145 Z4282 Escherichia coli O157:H7 str. EDL933 958414 15803476 Z4282 AE005174 NP_289509 hypothetical protein Virulence factor MUTATION: z4282 mutant is attenuated in calves (van et al., 2005). 15731074
2146 speA Escherichia coli 958416 15803477 Z4283 NP_289510.1 arginine decarboxylase Virulence factor MUTATION: speA mutant is attenuated in calves (van et al., 2005). 15731074
2147 Z4318 Escherichia coli O157:H7 str. EDL933 958457 15803511 Z4318 AE005174 NP_289544 hypothetical protein Virulence factor MUTATION: Z4318 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2148 pitB Escherichia coli O157:H7 str. EDL933 958466 15803532 Z4341 AE005174 NP_289565 partial putative transport protein Virulence factor MUTATION: pitB mutant is attenuated in calves (van et al., 2005). 15731074
2149 Z1126 Escherichia coli O157:H7 str. EDL933 958522 15800647 Z1126 AE005174 NP_286661 hypothetical protein Virulence factor MUTATION: Z1126 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2150 Z1129 Escherichia coli O157:H7 str. EDL933 958525 15800650 Z1129 AE005174 NP_286664 putative helicase Virulence factor MUTATION: z1129 mutant is attenuated in calves (van et al., 2005). 15731074
2151 tdcE Escherichia coli O157:H7 str. EDL933 958549 15803653 Z4466 AE005174 NP_289686 formate acetyltransferase 3 Virulence factor MUTATION: tdcE mutant is attenuated in calves (van et al., 2005). 15731074
2152 Z1182 Escherichia coli O157:H7 str. EDL933 958755 15800703 Z1182 AE005174 NP_286717 hypothetical protein Virulence factor MUTATION: Z1182 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2153 Z1202 Escherichia coli O157:H7 str. EDL933 958789 15800723 Z1202 AE005174 NP_286737 hypothetical protein Virulence factor MUTATION: Z1202 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2154 trxB Escherichia coli 958846 15800751 Z1232 NP_286765.1 thioredoxin reductase Virulence factor MUTATION: trxB mutant is attenuated in calves (van et al., 2005). 15731074
2155 ompA from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 958948 15800816 Z1307 NP_286832.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells (Prasadarao et al., 1999). 10531228
2156 Z1328 Escherichia coli O157:H7 str. EDL933 958973 15800834 Z1328 AE005174 NP_286850 hypothetical protein Virulence factor MUTATION: Z1328 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2157 yqeJ Escherichia coli O157:H7 str. EDL933 958999 15803368 Z4168 AE005174 NP_289401 hypothetical protein Virulence factor MUTATION: ygeJ mutant is attenuated in calves (Dziva et al., 2004). 15528651
2158 ymcC Escherichia coli O157:H7 str. EDL933 959050 15800907 Z1403 AE005174 NP_286923 putative regulator Virulence factor MUTATION: ymcC mutant is attenuated in calves (Dziva et al., 2004). 15528651
2159 stx2B from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 959107 15800961 Z1465 AE005174 NP_286977 shiga-like toxin II B subunit encoded by bacteriophage BP-933W Virulence factor TOXIN: Stx induces apoptosis in HeLa cells (Cherla et al., 2003). 14638419
2160 pgaA Escherichia coli O157:H7 str. EDL933 959162 15801015 Z1526 AE005174 NP_287031 outer membrane protein PgaA Virulence factor MUTATION: ycdS mutant is attenuated in calves (Dziva et al., 2004). 15528651
2161 agaB Escherichia coli O157:H7 str. EDL933 959220 15803678 Z4492 AE005174 NP_289711 PTS system N-acetylgalactosamine-specific transporter subunit IIB Virulence factor MUTATION: agaB mutant is attenuated in calves (Dziva et al., 2004). 15528651
2162 mdoC Escherichia coli O157:H7 str. EDL933 959379 15801164 Z1681 AE005174 NP_287181 glucans biosynthesis protein Virulence factor MUTATION: mdoC (ymdD) mutant is attenuated in calves (van et al., 2005). 15731074
2163 ypjA Escherichia coli O157:H7 str. EDL933 959506 15803170 Z3948 AE005174 NP_289202 putative ATP-binding component of a transport system Virulence factor MUTATION: ypjA mutant is attenuated in calves (Dziva et al., 2004). 15528651
2164 serA Escherichia coli O157:H7 str. EDL933 959584 15803448 Z4251 AE005174 NP_289481 D-3-phosphoglycerate dehydrogenase Virulence factor MUTATION: serA mutant is attenuated in calves (van et al., 2005). 15731074
2165 Z1829 Escherichia coli O157:H7 str. EDL933 959588 15801299 Z1829 AE005174 NP_287316 hypothetical protein Virulence factor MUTATION: z1829 mutant is attenuated in calves (van et al., 2005). 15731074
2166 Z5946 Escherichia coli O157:H7 str. EDL933 959707 15804920 Z5946 AE005174 NP_290962 putative restriction modification enzyme S subunit Virulence factor MUTATION: Z5946 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2167 fimH from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 959733 15804895 Z5918 NP_290936.1 minor fimbrial subunit, D-mannose specific adhesin Virulence factor MUTATION: attenuated in mice and humans (Wiles et al., 2008). 18482721
2168 fimE Escherichia coli O157:H7 str. EDL933 959740 15804888 Z5911 AE005174 NP_290929 tyrosine recombinase Virulence factor MUTATION: fimE mutant is attenuated in calves (van et al., 2005). 15731074
2169 yjhS Escherichia coli O157:H7 str. EDL933 959744 15804884 Z5905 AE005174 NP_290925 hypothetical protein Virulence factor MUTATION: yjhS mutant is attenuated in calves (Dziva et al., 2004). 15528651
2170 Z5886 Escherichia coli O157:H7 str. EDL933 959763 15804865 Z5886 AE005174 NP_290906 hypothetical protein Virulence factor MUTATION: Z5886 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2171 nrdD Escherichia coli O157:H7 str. EDL933 959802 15804828 Z5848 AE005174 NP_290869 anaerobic ribonucleoside triphosphate reductase Virulence factor MUTATION: nrdD mutant is attenuated in calves (van et al., 2005). 15731074
2172 Z1930 Escherichia coli O157:H7 str. EDL933 959880 15801390 Z1930 AE005174 NP_287407 putative protease encoded within prophage CP-933X Virulence factor MUTATION: Z1930 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2173 yjeJ Escherichia coli O157:H7 str. EDL933 959978 15804737 Z5750 AE005174 NP_290778 hypothetical protein Virulence factor MUTATION: yjeJ mutant is attenuated in calves (Dziva et al., 2004). 15528651
2174 yjcF Escherichia coli O157:H7 str. EDL933 960058 15804658 Z5665 AE005174 NP_290699 hypothetical protein Virulence factor MUTATION: yjcF mutant is attenuated in calves (Dziva et al., 2004). 15528651
2175 arp Escherichia coli O157:H7 str. EDL933 960113 15804603 Z5608 AE005174 NP_290644 regulator of acetyl CoA synthetase Virulence factor MUTATION: arp mutant is attenuated in calves (Dziva et al., 2004). 15528651
2176 Z5444 Escherichia coli O157:H7 str. EDL933 960248 15804490 Z5444 AE005174 NP_290530 putative glycoporin Virulence factor MUTATION: Z5444 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2177 dsbA from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 960300 15804445 Z5392 NP_290485.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC (Donnenberg et al., 1997). 9224871
2178 rfaH from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 960318 15804432 Z5362 NP_290472.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice (Nagy et al., 2002). 12117951
2179 ilvG Escherichia coli O157:H7 str. EDL933 960401 15804359 Z5279 AE005174 NP_290399 acetolactate synthase 2 catalytic subunit Virulence factor MUTATION: ilvG mutant is attenuated in calves (Dziva et al., 2004). 15528651
2180 narG Escherichia coli O157:H7 str. EDL933 960477 15801455 Z2001 AE005174 NP_287472 nitrate reductase 1, alpha subunit Virulence factor MUTATION: narG mutant is attenuated in calves (van et al., 2005). 15731074
2181 tonB from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 960504 15801478 Z2030 NP_287495.1 membrane spanning protein in TonB-ExbB-ExbD complex; transduces proton motive force of the cytoplasmic membrane to outer membrane transporters; involved in the transport of ron-siderophore complexes, vitamin B12 and colicins Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
2182 Z2056 Escherichia coli O157:H7 str. EDL933 960527 15801501 Z2056 AE005174 NP_287518 hypothetical protein Virulence factor MUTATION: z2056 mutant is attenuated in calves (van et al., 2005). 15731074
2183 Z2142 Escherichia coli O157:H7 str. EDL933 960608 15801579 Z2142 AE005174 NP_287596 putative tail component of prophage CP-933O Virulence factor MUTATION: z2142 mutant is attenuated in calves (van et al., 2005). 15731074
2184 Z2144 Escherichia coli O157:H7 str. EDL933 960610 15801581 Z2144 AE005174 NP_287598 putative tail component of prophage CP-933O Virulence factor MUTATION: Z2144 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2185 Z2149 Escherichia coli 960615 15801586 Z2149 NP_287603.1 hypothetical protein Virulence factor MUTATION: Z2149 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2186 Z2176 Escherichia coli O157:H7 str. EDL933 960638 15801609 Z2176 AE005174 NP_287626 hypothetical protein Virulence factor MUTATION: z2176 mutant is attenuated in calves (van et al., 2005). 15731074
2187 ydeK Escherichia coli O157:H7 str. EDL933 960656 15801627 Z2195 AE005174 NP_287644 putative lipoprotein Virulence factor MUTATION: ydeK mutant is attenuated in calves (van et al., 2005). 15731074
2188 Z2203 Escherichia coli O157:H7 str. EDL933 960662 15801633 Z2203 AE005174 NP_287650 putative fimbrial usher protein Virulence factor MUTATION: Z2203 mutation is attenuated in calves (Dziva et al., 2004). 15528651
2189 Z2205 Escherichia coli O157:H7 str. EDL933 960664 15801635 Z2205 AE005174 NP_287652 putative fimbrial-like protein Virulence factor MUTATION: z2205 mutant is attenuated in calves (van et al., 2005). 15731074
2190 Z2207 Escherichia coli O157:H7 str. EDL933 960666 15801637 Z2207 AE005174 NP_287654 putative oxidoreductase Virulence factor MUTATION: z2207 mutant is attenuated in calves (van et al., 2005). 15731074
2191 yddA Escherichia coli O157:H7 str. EDL933 960672 15801642 Z2212 AE005174 NP_287659 putative ATP-binding component of a transport system Virulence factor MUTATION: yddA mutant is attenuated in calves (van et al., 2005). 15731074
2192 Z5214 Escherichia coli O157:H7 str. EDL933 960719 15804316 Z5214 AE005174 NP_290355 hypothetical protein Virulence factor MUTATION: Z5214 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2193 Z5212 Escherichia coli O157:H7 str. EDL933 960725 15804314 Z5212 AE005174 NP_290353 hypothetical protein Virulence factor MUTATION: Z5212 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2194 Z5131 Escherichia coli O157:H7 str. EDL933 960841 15804240 Z5131 AE005174 NP_290279 hypothetical protein Virulence factor MUTATION: Z5131 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2195 escC Escherichia coli O157:H7 str. EDL933 960846 15804236 Z5126 AE005174 NP_290275 hypothetical protein Virulence factor MUTATION: escC mutant is attenuated in calves (Dziva et al., 2004). 15528651
2196 Z5121 Escherichia coli O157:H7 str. EDL933 960851 15804231 Z5121 AE005174 NP_290270 hypothetical protein Virulence factor MUTATION: z5121(st22) mutant is attenuated in calves (van et al., 2005). 15731074
2197 escV Escherichia coli O157:H7 str. EDL933 960852 15804230 Z5120 AE005174 NP_290269 hypothetical protein Virulence factor MUTATION: escV mutant is attenuated in calves (Dziva et al., 2004). 15528651
2198 escN from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 960853 15804229 Z5119 NP_290268.1 escN Virulence factor MUTATION: deletion of escN abolished pathogenicity in infant rabbits compared to wild-type EHEC (Ritchie and Waldor, 2005). 15731044
2199 sepQ Escherichia coli O157:H7 str. EDL933 960856 15804226 Z5116 AE005174 NP_290265 hypothetical protein Virulence factor MUTATION: sepQ mutant is attenuated in calves (Dziva et al., 2004). 15528651
2200 Z5114 Escherichia coli O157:H7 str. EDL933 960858 15804224 Z5114 AE005174 NP_290263 hypothetical protein Virulence factor MUTATION: Z5114 (cesF) mutant is attenuated in calves (Dziva et al., 2004). 15528651
2201 tir from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 960860 15804222 Z5112 NP_290261.1 putative translocated intimin receptor protein Virulence factor MUTATION: tir deletion strains are unable to adhere to tissue in an in vitro organ culture (Schüller et al., 2007). 17474908
2202 eae from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 960862 15804220 Z5110 NP_290259.1 intimin adherence protein Virulence factor MUTATION: attenuated in humans (Donnenberg et al., 1993). 8376594
2203 espA from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 960865 15804217 Z5107 NP_290256.1 secreted protein EspA Virulence factor MUTATION: deletion of espA in STEC abolished ability to attach to HeLa cells and induce actin rearrangements (Ebel et al., 1998). 9786192
2204 espD from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 960866 15804216 Z5106 NP_290255.1 secreted protein EspD Virulence factor MUTATION: espD deletion mutant of the enterohemorrhagic E. coli (EHEC) strain EDL933 had impaired attachment to HeLa cells (Kresse et al., 1999). 10456938
2205 espB from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 960867 15804215 Z5105 NP_290254.1 secreted protein EspB Virulence factor MUTATION: deletion mutant attenuated in humans compared to wild-type EPEC (Tacket et al., 2000). 10816529
2206 espF from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 960871 15804211 Z5100 NP_290250.1 espF Virulence factor MUTATION: espF EPEC mutant is deficient in its ability to disrupt electrical resistance across a polarized monolayer of intestinal epithelial cells, suggesting that EspF is an effector molecule required for disrupting intestinal epithelial barrier function (McNamara et al., 2001). 11238563
2207 yibD Escherichia coli 960931 15804159 Z5042 NP_290198.1 predicted glycosyl transferase Virulence factor MUTATION: yibD mutant is attenuated in calves (Dziva et al., 2004). 15528651
2208 rhsA Escherichia coli O157:H7 str. EDL933 960959 15804134 Z5014 AE005174 NP_290173 rhsA protein in rhs element Virulence factor MUTATION: rhsA mutant is attenuated in calves (van et al., 2005). 15731074
2209 hrpA Escherichia coli O157:H7 str. EDL933 961000 15801734 Z2313 AE005174 NP_287752 ATP-dependent RNA helicase HrpA Virulence factor MUTATION: hrpA mutant is attenuated in calves (Dziva et al., 2004). 15528651
2210 Z2318 Escherichia coli O157:H7 str. EDL933 961004 15801738 Z2318 AE005174 NP_287756 putative phosphatidate cytidiltransferase Virulence factor MUTATION: Z2318 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2211 dppA from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 961074 15804087 Z4961 NP_290125.1 dipeptide transport protein Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice (Haugen et al., 2007). 17074858
2212 dctA Escherichia coli O157:H7 str. EDL933 961092 15804072 Z4942 AE005174 NP_290108 C4-dicarboxylate transporter DctA Virulence factor MUTATION: dctA mutant is attenuated in calves (Dziva et al., 2004). 15528651
2213 Z2368 Escherichia coli O157:H7 str. EDL933 961108 15801782 Z2368 AE005174 NP_287800 hypothetical protein Virulence factor MUTATION: Z2368 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2214 chuA from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 961176 15804044 Z4911 NP_290080.1 outer membrane heme/hemoglobin receptor Virulence factor MUTATION: chuA mutant unable to grow on iron-depleted medium containing heme (Torres and Payne, 1997). 9157252
2215 yhiJ Escherichia coli 961212 15804024 Z4887 NP_290060.1 hypothetical protein Virulence factor MUTATION: yhiJ mutant is attenuated in calves (Dziva et al., 2004). 15528651
2216 Z3620 Escherichia coli O157:H7 str. EDL933 961230 15802901 Z3620 AE005174 NP_288928 hypothetical protein Virulence factor MUTATION: Z3620 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2217 ycjV Escherichia coli O157:H7 str. EDL933 961311 15801852 Z2463 AE005174 NP_287870 putative ATP-binding component of a transport system Virulence factor MUTATION: ycjV mutant is attenuated in calves (van et al., 2005). 15731074
2218 acnA Escherichia coli O157:H7 str. EDL933 961382 15801901 Z2532 AE005174 NP_287921 aconitate hydratase Virulence factor MUTATION: acnA mutant is attenuated in calves (van et al., 2005). 15731074
2219 trpB Escherichia coli O157:H7 str. EDL933 961399 15801917 Z2550 AE005174 NP_287938 tryptophan synthase subunit beta Virulence factor MUTATION: trpB mutant is attenuated in calves (van et al., 2005). 15731074
2220 Z6010 Escherichia coli O157:H7 str. EDL933 961413 15801929 Z6010 AE005174 NP_287950 hypothetical protein Virulence factor MUTATION: Z6010 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2221 trkA Escherichia coli O157:H7 str. EDL933 961474 15803817 Z4660 AE005174 NP_289851 potassium transporter peripheral membrane component Virulence factor MUTATION: trkA mutant is attenuated in calves (van et al., 2005). 15731074
2222 Z2702 Escherichia coli O157:H7 str. EDL933 961645 15802086 Z2702 AE005174 NP_288108 hypothetical protein Virulence factor MUTATION: Z2702 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2223 Z2749 Escherichia coli O157:H7 str. EDL933 961691 15802132 Z2749 AE005174 NP_288154 hypothetical protein Virulence factor MUTATION: z2749 mutant is attenuated in calves (van et al., 2005). 15731074
2224 Z2751 Escherichia coli O157:H7 str. EDL933 961692 15802133 Z2751 AE005174 NP_288155 hypothetical protein Virulence factor MUTATION: z2751 mutant is attenuated in calves (van et al., 2005). 15731074
2225 celF Escherichia coli O157:H7 str. EDL933 961704 15802145 Z2764 AE005174 NP_288167 6-phospho-beta-glucosidase Virulence factor MUTATION: celF mutant is attenuated in calves (Dziva et al., 2004). 15528651
2226 cspC Escherichia coli O157:H7 str. EDL933 961796 15802236 Z2868 AE005174 NP_288259 cold shock-like protein CspC Virulence factor MUTATION: cspC mutant is attenuated in calves (Dziva et al., 2004). 15528651
2227 Z2984 Escherichia coli O157:H7 str. EDL933 961896 15802336 Z2984 AE005174 NP_288362 putative serine acetlyltransferase of prophage CP-933T Virulence factor MUTATION: Z2984 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2228 sdiA Escherichia coli O157:H7 str. EDL933 961914 15802351 Z3004 AE005174 NP_288377 DNA-binding transcriptional activator SdiA Virulence factor MUTATION: sidA mutant is attenuated in calves (Dziva et al., 2004). 15528651
2229 fliC from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 961921 15802358 Z3013 NP_288384.1 flagellin Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains (Erdem et al., 2007). 17693516
2230 yedL Escherichia coli 961931 15802367 Z3022 NP_288393.1 hypothetical protein Virulence factor MUTAITON: yedL mutant is attenuated in calves (Dziva et al., 2004). 15528651
2231 Z3023 Escherichia coli O157:H7 str. EDL933 961932 15802368 Z3023 AE005174 NP_288394 putative secreted protein Virulence factor MUTATION: Z3023 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2232 Z3026 Escherichia coli O157:H7 str. EDL933 961934 15802370 Z3026 GN097348 NP_288396 putative secreted protein Virulence factor MUTATION: Z3026 mutant is attenuated in calves (Dziva et al., 2004). 15528651
2233 Z3062 Escherichia coli O157:H7 str. EDL933 961974 15802403 Z3062 AE005174 NP_288429 hypothetical protein Virulence factor MUTATION: z3062 mutant is attenuated in calves (van et al., 2005). 15731074
2234 manB from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 962089 15802510 Z3194 NP_288536.1 phosphomannomutase Virulence factor MUTATION: manB mutant is attenuated in calves (Dziva et al., 2004). 15528651
2235 manC Escherichia coli O157:H7 str. EDL933 962090 15802511 Z3195 AE005174 NP_288537 mannose-1-P guanosyltransferase Virulence factor MUTATION: manC mutant is attenuated in calves (Dziva et al., 2004). 15528651
2236 fcI Escherichia coli O157:H7 str. EDL933 962092 15802513 Z3197 AE005174 NP_288539 fucose synthetase Virulence factor MUTATION: fcl mutant is attenuated in calves (Dziva et al., 2004). 15528651
2237 wbdP Escherichia coli O157:H7 str. EDL933 962094 15802515 Z3199 AE005174 NP_288541 glycosyl transferase Virulence factor MUTATION: wbdP mutant is attenuated in calves (Dziva et al., 2004). 15528651
2238 per Escherichia coli O157:H7 str. EDL933 962095 15802516 Z3200 AE005174 NP_288542 perosamine synthetase Virulence factor MUTATION: per mutant is attenuated in calves (Dziva et al., 2004). 15528651
2239 iutA from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6143094 170680936 EcSMS35_3178 YP_001745168.1 ferric aerobactin receptor IutA Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
2240 rfaH from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6143458 170682425 EcSMS35_4222 YP_001746174.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice (Nagy et al., 2002). 12117951
2241 iucB from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6144084 170681805 EcSMS35_3181 YP_001745171.1 aerobactin siderophore biosynthesis protein IucB Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
2242 dsbA from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6144184 170681207 EcSMS35_4241 YP_001746187.1 thiol:disulfide interchange protein DsbA Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC (Donnenberg et al., 1997). 9224871
2243 dppA from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6144283 170679624 EcSMS35_3863 YP_001745830.1 dipeptide ABC transporter, periplasmic dipeptide-binding protein Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice (Haugen et al., 2007). 17074858
2244 fliC from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6144795 170682489 EcSMS35_1258 YP_001743319.1 flagellin FliC Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains (Erdem et al., 2007). 17693516
2245 tonB from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6145010 170681605 EcSMS35_1880 YP_001743934.1 outer membrane receptor-mediated transport energizer protein TonB Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
2246 iucD from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6145117 170682870 EcSMS35_3179 YP_001745169.1 L-lysine 6-monooxygenase IucD Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
2247 fimH from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6145732 170681950 EcSMS35_4847 YP_001746758.1 protein FimH Virulence factor MUTATION: attenuated in mice and humans (Wiles et al., 2008). 18482721
2248 iucA from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6145998 170683655 EcSMS35_3182 YP_001745172.1 aerobactin siderophore biosynthesis protein IucA Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
2249 aslA from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6146022 170681296 EcSMS35_4166 YP_001746117.1 arylsulfatase Virulence factor MUTATION: disruption mutant was less invasive in an in vitro HBMEC invasion assay than the wild-type E. coli K1 (Hoffman et al., 2000). 10948126
2250 ompA from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6146246 170681860 EcSMS35_2163 YP_001744213.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells (Prasadarao et al., 1999). 10531228
2251 ibeA from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6146360 170682712 EcSMS35_4868 YP_001746779.1 invasion protein IbeA Virulence factor MUTATION: deletion mutant was significantly less invasive in human brain microvascular endothelial cells (BMECs) than the parent strain (E. coli K1) (Huang et al., 2001). 11237832
2252 chuA from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6146648 170681293 EcSMS35_3802 YP_001745768.1 outer membrane heme/hemoglobin receptor ChuA Virulence factor MUTATION: chuA mutant unable to grow on iron-depleted medium containing heme (Torres and Payne, 1997). 9157252
2253 iucC from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6147083 170679922 EcSMS35_3180 YP_001745170.1 aerobactin siderophore biosynthesis protein IucC Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
2254 traJ from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6106483 170650783 EcSMS35_A0041 YP_001739929.1 type IV secretion-like conjugative transfer system regulator TraJ Virulence factor MUTATION: traJ mutants of E. coli K1 are attenuated in rat pups and macrophages (Hill et al., 2004). 14688129
2255 traT from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6106527 170650825 EcSMS35_A0012 YP_001739900.1 type IV secretion-like conjugative transfer system complement resistance protein TraT Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics (Sukupolvi and O'Connor, 1987). 3323837
2256 ompA from Escherichia coli str. K-12 substr. MG1655 Escherichia coli str. K-12 substr. MG1655 945571 16128924 b0957 NP_415477.1 outer membrane protein A (3a;II*;G;d) Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells (Prasadarao et al., 1999). 10531228
2257 tonB from Escherichia coli str. K-12 substr. MG1655 Escherichia coli str. K-12 substr. MG1655 945843 16129213 b1252 NP_415768.1 membrane spanning protein in TonB-ExbB-ExbD complex Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
2258 cusC Escherichia coli str. K-12 substr. MG1655 946288 16128555 b0572 U00096 NP_415104 copper/silver efflux system, outer membrane component Virulence factor MUTATION: ibeB mutant is unable to invade BMEC and was attenuated in a newborn rat model (Huang et al., 1999). 10225861
2259 dppA from Escherichia coli str. K-12 substr. MG1655 Escherichia coli str. K-12 substr. MG1655 948062 16131416 b3544 NP_418001.1 dipeptide transporter Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice (Haugen et al., 2007). 17074858
2260 rfaL Escherichia coli str. K-12 substr. MG1655 948148 16131493 b3622 U00096 NP_418079 O-antigen ligase Virulence factor MUTATION: rfaL mutant is attenuated in rats (Gonzalez et al., 2001). 11430402
2261 setC Escherichia coli str. K-12 substr. MG1655 948177 16131529 b3659 U00096 NP_418115 predicted sugar efflux system Virulence factor MUTATION: yicK mutant is attenuated in calves (van et al., 2005). 15731074
2262 rfaH from Escherichia coli str. K-12 substr. MG1655 Escherichia coli str. K-12 substr. MG1655 948327 16131688 b3842 NP_418284.1 DNA-binding transcriptional antiterminator Virulence factor MUTATION: attenuated in mice (Nagy et al., 2002). 12117951
2263 dsbA from Escherichia coli str. K-12 substr. MG1655 Escherichia coli str. K-12 substr. MG1655 948353 16131701 b3860 NP_418297.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC (Donnenberg et al., 1997). 9224871
2264 yijP Escherichia coli str. K-12 substr. MG1655 948458 16131793 b3955 U00096 NP_418390 conserved inner membrane protein Virulence factor MUTATION: yijP mutant had reduced invasion of BMEC (Wang et al., 1999). 10456927
2265 fimH from Escherichia coli str. K-12 substr. MG1655 Escherichia coli str. K-12 substr. MG1655 948847 16132141 b4320 NP_418740.1 minor component of type 1 fimbriae Virulence factor MUTATION: attenuated in humans and mice (Wiles et al., 2008). 18482721
2266 aslA from Escherichia coli str. K-12 substr. MG1655 Escherichia coli str. K-12 substr. MG1655 949015 16131653 b3801 NP_418245.1 acrylsulfatase-like enzyme Virulence factor MUTATION: disruption mutant was less invasive in an in vitro HBMEC invasion assay than the wild-type E. coli K1 (Hoffman et al., 2000). 10948126
2267 fliC from Escherichia coli str. K-12 substr. MG1655 Escherichia coli str. K-12 substr. MG1655 949101 16129870 b1923 NP_416433.1 flagellar filament structural protein (flagellin) Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains (Erdem et al., 2007). 17693516
2268 dsbA from Escherichia coli str. K-12 substr. DH10B Escherichia coli str. K-12 substr. DH10B 6058256 170083333 ECDH10B_4049 YP_001732653.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC (Donnenberg et al., 1997). 9224871
2269 tonB from Escherichia coli str. K-12 substr. DH10B Escherichia coli str. K-12 substr. DH10B 6058282 170080929 ECDH10B_1367 YP_001730249.1 membrane spanning protein in TonB-ExbB-ExbD complex Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
2270 dppA from Escherichia coli str. K-12 substr. DH10B Escherichia coli str. K-12 substr. DH10B 6058698 170083053 ECDH10B_3723 YP_001732373.1 dipeptide transporter; periplasmic-binding component of ABC superfamily Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice (Haugen et al., 2007). 17074858
2271 rfaH from Escherichia coli str. K-12 substr. DH10B Escherichia coli str. K-12 substr. DH10B 6058984 170083320 ECDH10B_4031 YP_001732640.1 DNA-binding transcriptional antiterminator Virulence factor MUTATION: attenuated in mice (Nagy et al., 2002). 12117951
2272 ompA from Escherichia coli str. K-12 substr. DH10B Escherichia coli str. K-12 substr. DH10B 6060328 170080615 ECDH10B_1027 YP_001729935.1 outer membrane protein A (3a;II*;G;d) Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells (Prasadarao et al., 1999). 10531228
2273 aslA from Escherichia coli str. K-12 substr. DH10B Escherichia coli str. K-12 substr. DH10B 6061059 170083285 ECDH10B_3991 YP_001732605.1 acrylsulfatase-like enzyme Virulence factor MUTATION: disruption mutant was less invasive in an in vitro HBMEC invasion assay than the wild-type E. coli K1 (Hoffman et al., 2000). 10948126
2274 toxB from Escherichia coli O157:H7 str. Sakai Escherichia coli O157:H7 str. Sakai 1789667 10955324 pO157p58 NP_052665.1 toxin B Virulence factor MUTATION: toxB mutants had attenuated adherence to Caco-2 cells (Tatsuno et al., 2001). 11598035
2275 hlyA from Escherichia coli O157:H7 str. Sakai Escherichia coli O157:H7 str. Sakai 1789686 10955283 pO157p18 NP_052624.1 hemolysin A Virulence factor MUTATION: attenuated in zebra fish (Wiles et al., 2009). 20019794
2276 hlyC from Escherichia coli O157:H7 str. Sakai Escherichia coli O157:H7 str. Sakai 1789689 10955282 pO157p17 NP_052623.1 hemolysin C Virulence factor MUTATION: hlyC is required for the acylation and therefore activation of the toxin hemolysin which has the ability to lyse several different types of cells (Stanley et al., 1996). 8793877
2277 espP from Escherichia coli O157:H7 str. Sakai Escherichia coli O157:H7 str. Sakai 1789732 10955344 pO157p78 NP_052685.1 EspP Virulence factor MUTATION: espP mutant is attenuated in calves (Dziva et al., 2007). 17451446
2278 traJ from Escherichia coli APEC O1 Escherichia coli APEC O1 5616535 157418110 APECO1_O1CoBM27 YP_001481182.1 positive regulator of conjugative transfer; similar to AAO49515 Virulence factor MUTATION: traJ mutants of E. coli K1 are attenuated in rat pups and macrophages (Hill et al., 2004). 14688129
2279 traT from Escherichia coli APEC O1 Escherichia coli APEC O1 5616563 157418138 APECO1_O1CoBM55 YP_001481210.1 surface exclusion and complement resistance protein; similar to AAT85681; identified by match to protein family HMM PF05818 Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics (Sukupolvi and O'Connor, 1987). 3323837
2280 tsh Escherichia coli APEC O1 5616581 157418156 APECO1_O1CoBM73 DQ381420 YP_001481228 Tsh Virulence factor MUTATION: tsh mutant is attenuated in chickens (Chen et al., 2008). 18589815
2281 iroN from Escherichia coli APEC O1 Escherichia coli APEC O1 5616645 157418221 APECO1_O1CoBM137 YP_001481293.1 salmochelin siderophore system, salmochelin receptor; similar to AAS80269; identified by match to protein family HMM PF00593 Virulence factor MUTATION: iroA gene cluster mutants were unable to resist Lcn2 in mouse serum (Fischbach et al., 2006). 17060628
2282 iroD from Escherichia coli APEC O1 Escherichia coli APEC O1 5616647 157418223 APECO1_O1CoBM139 YP_001481295.1 salmochelin siderophore system, ferric enterochelin esterase; similar to AAS80267; identified by match to protein family HMM PF Virulence factor MUTATION: attenuated in chicks (Caza et al., 2008). 18541653
2283 iroC from Escherichia coli APEC O1 Escherichia coli APEC O1 5616648 157418224 APECO1_O1CoBM140 YP_001481296.1 salmochelin siderophore system, ATP-binding cassette; similar to AAP41471; identified by match to protein family HMM PF00005 Virulence factor MUTATION: attenuated in chicks (Caza et al., 2008). 18541653
2284 iroB from Escherichia coli APEC O1 Escherichia coli APEC O1 5616649 157418225 APECO1_O1CoBM141 YP_001481297.1 salmochelin siderophore system, glycosyltransferase; similar to AAS80265; identified by match to protein family HMM PF06722 Virulence factor MUTATION: attenuated in chicks (Caza et al., 2008). 18541653
2285 iutA from Escherichia coli APEC O1 Escherichia coli APEC O1 5616676 157418252 APECO1_O1CoBM168 YP_001481324.1 aerobactin siderophore system, ferric aerobactin receptor protein; similar to ABA54744; identified by match to protein family HMM PF00593 Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
2286 iucD from Escherichia coli APEC O1 Escherichia coli APEC O1 5616677 157418253 APECO1_O1CoBM169 YP_001481325.1 aerobactin siderophore system, L-lysine 6-monooxygenase; similar to CAE55774 Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
2287 iucC from Escherichia coli APEC O1 Escherichia coli APEC O1 5616678 157418254 APECO1_O1CoBM170 YP_001481326.1 aerobactin siderophore system, aerobactin biosynthesis protein; similar to ABA54742; identified by match to protein family HMMPFO4183 Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
2288 iucA from Escherichia coli APEC O1 Escherichia coli APEC O1 5616680 157418256 APECO1_O1CoBM172 YP_001481328.1 aerobactin siderophore system, aerobactin synthetase; similar to ABA54740; identified by match to protein family HMM PF04183 Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
2289 eatA from Escherichia coli E24377A Escherichia coli E24377A 5585855 157149562 EcE24377A_E0051 YP_001451588.1 secreted serine peptidase EatA Virulence factor MUTATION: interruption of the eatA gene retarded fluid accumulation in the rabbit ileal loop model, suggesting that this autotransporter contributes to the virulence of ETEC (Patel et al., 2004). 14977988
2290 traJ from Escherichia coli UTI89 Escherichia coli UTI89 3996471 91206349 UTI89_P104 YP_538703.1 functional relaxosome complex initiator Virulence factor MUTATION: traJ mutants of E. coli K1 are attenuated in rat pups and macrophages (Hill et al., 2004). 14688129
2291 traT from Escherichia coli UTI89 Escherichia coli UTI89 3996502 91206380 UTI89_P135 YP_538734.1 surface exclusion outer membrane protein Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics (Sukupolvi and O'Connor, 1987). 3323837
2292 espP from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 3683719 75994466 L7020 YP_325580.1 putative exoprotein-precursor Virulence factor MUTATION: espP mutant is attenuated in calves (Dziva et al., 2007). 17451446
2293 EHEC-hlyC Escherichia coli O157:H7 str. EDL933 3683746 75994493 L7047 AF074613 YP_325607 hemolysin transport protein Virulence factor MUTATION: hlyC is required for the acylation and therefore activation of the toxin hemolysin which has the ability to lyse several different types of cells (Stanley et al., 1996). 8793877
2294 EHEC-hlyA Escherichia coli O157:H7 str. EDL933 3683747 75994494 L7048 AF074613 YP_325608 hemolysin toxin protein Virulence factor MUTATION: hlyA mutant is attenuated in calves (van et al., 2005). 15731074
2295 traT Escherichia coli 2716459 41057009 pC15-1a_084 FJ449539 NP_957629 conjugal transfer surface exclusion protein TraT Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics (Sukupolvi and O'Connor, 1987). 3323837
2296 tir Escherichia coli 2716469 41057025 pC15-1a_100 AF550679 NP_957645 hypothetical protein Virulence factor MUTATION: tir deletion strains are unable to adhere to tissue in an in vitro organ culture (Schüller et al., 2007). 17474908
2297 traT Escherichia coli 1446509 32470006 p165897_034 NP_862946.1 TraT Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics (Sukupolvi and O'Connor, 1987). 3323837
2298 tir Escherichia coli 1446522 32470019 p165897_049 NP_862961.1 Tir Virulence factor MUTATION: tir deletion strains are unable to adhere to tissue in an in vitro organ culture (Schüller et al., 2007). 17474908
2299 traT from Escherichia coli E24377A Escherichia coli E24377A 5585733 157149414 EcE24377A_C0002 YP_001451443.1 TraT complement resistance protein Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics (Sukupolvi and O'Connor, 1987). 3323837
2300 eltA from Escherichia coli E24377A Escherichia coli E24377A 5585630 157149353 EcE24377A_F0020 YP_001451390.1 heat-labile enterotoxin A Virulence factor MUTATION: Deletion mutants of eltA and eltB had decreased levels of inducing cAMP secretion in an in vitro culture system, and had less adherence to IPEC-J2 and Caco-2 cells than the wild-type ETEC (Johnson et al., 2009). 18978047
3368 rfaH from Escherichia coli SE11 Escherichia coli SE11 7002513 209921319 ECSE_4128 YP_002295403.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice (Nagy et al., 2002). 12117951
3369 tonB from Escherichia coli O157:H7 str. EC 4115 Escherichia coli O157:H7 str. EC 4115 6967035 209396499 ECH74115_1739 YP_002270184.1 transport protein TonB Virulence factor MUTATION: attenuated in mice (Torres et al., 2001) 11553558
3370 dsbA from Escherichia coli O157:H7 str. EC 4115 Escherichia coli O157:H7 str. EC 4115 6967123 209400816 ECH74115_5300 YP_002273372.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC (Donnenberg et al., 1997). 9224871
3371 dppA from Escherichia coli O157:H7 str. EC 4115 Escherichia coli O157:H7 str. EC 4115 6967851 209396782 ECH74115_4912 YP_002273027.1 dipeptide ABC transporter, periplasmic dipeptide-binding protein Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice (Haugen et al., 2007). 17074858
3372 chuA from Escherichia coli O157:H7 str. EC 4115 Escherichia coli O157:H7 str. EC 4115 6967853 209396568 ECH74115_4857 YP_002272975.1 outer membrane heme/hemoglobin receptor ChuA Virulence factor MUTATION: chuA mutant unable to grow on iron-depleted medium containing heme (Torres and Payne, 1997). 9157252
3373 rfaH from Escherichia coli O157:H7 str. EC 4115 Escherichia coli O157:H7 str. EC 4115 6968671 209396563 ECH74115_5280 YP_002273359.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice (Nagy et al., 2002). 12117951
3374 ompA from Escherichia coli O157:H7 str. EC 4115 Escherichia coli O157:H7 str. EC 4115 6969157 209397392 ECH74115_1121 YP_002269625.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells (Prasadarao et al., 1999). 10531228
3375 fimH from Escherichia coli O157:H7 str. EC 4115 Escherichia coli O157:H7 str. EC 4115 6969617 209399868 ECH74115_5826 YP_002273845.1 protein FimH Virulence factor MUTATION: attenuated in mice and humans (Wiles et al., 2008). 18482721
3463 iroN from Escherichia coli (strain: chi7122, serotype: O78:K80:H9, group: ExPEC, subgroup: APEC) Escherichia coli (strain: chi7122, serotype: O78:K80:H9, group: ExPEC, subgroup: APEC) 7379404 222104801 MM1_0014 YP_002539290.1 IroN, siderophore receptor Virulence factor MUTATION: iroA gene cluster mutants were unable to resist Lcn2 in mouse serum (Fischbach et al., 2006). 17060628
3464 iroD from Escherichia coli (strain: chi7122, serotype: O78:K80:H9, group: ExPEC, subgroup: APEC) Escherichia coli (strain: chi7122, serotype: O78:K80:H9, group: ExPEC, subgroup: APEC) 7379484 222104799 MM1_0011 YP_002539288.1 IroD, ferric enterochelin esterase Virulence factor MUTATION: attenuated in chicks (Caza et al., 2008). 18541653
3465 iutA from Escherichia coli (strain: chi7122, serotype: O78:K80:H9, group: ExPEC, subgroup: APEC) Escherichia coli (strain: chi7122, serotype: O78:K80:H9, group: ExPEC, subgroup: APEC) 7379559 222104883 MM1_0096 YP_002539372.1 ferric aerobactin receptor precursor Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
3466 iucC from Escherichia coli (strain: chi7122, serotype: O78:K80:H9, group: ExPEC, subgroup: APEC) Escherichia coli (strain: chi7122, serotype: O78:K80:H9, group: ExPEC, subgroup: APEC) 7379561 222104885 MM1_0098 YP_002539374.1 aerobactin siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3467 iucB from Escherichia coli (strain: chi7122, serotype: O78:K80:H9, group: ExPEC, subgroup: APEC) Escherichia coli (strain: chi7122, serotype: O78:K80:H9, group: ExPEC, subgroup: APEC) 7379562 222104886 MM1_0099 YP_002539375.1 IucB Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3468 iucA from Escherichia coli (strain: chi7122, serotype: O78:K80:H9, group: ExPEC, subgroup: APEC) Escherichia coli (strain: chi7122, serotype: O78:K80:H9, group: ExPEC, subgroup: APEC) 7379563 222104887 MM1_0100 YP_002539376.1 aerobactin siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3469 iucA from Escherichia coli (strain: APEC O103) Escherichia coli (strain: APEC O103) 7324506 221218526 pO103_24 YP_002527484.1 Aerobactin siderophore biosynthesis protein IucA Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3470 iucB from Escherichia coli (strain: APEC O103) Escherichia coli (strain: APEC O103) 7324507 221218527 pO103_25 YP_002527485.1 Aerobactin siderophore biosynthesis protein IucB Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3471 iucC from Escherichia coli (strain: APEC O103) Escherichia coli (strain: APEC O103) 7324508 221218528 pO103_26 YP_002527486.1 Aerobactin siderophore biosynthesis protein IucC Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3472 iucD from Escherichia coli (strain: APEC O103) Escherichia coli (strain: APEC O103) 7324509 221218529 pO103_27 YP_002527487.1 Aerobactin siderophore L-lysine 6-monooxygenase IucD Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3473 iutA from Escherichia coli (strain: APEC O103) Escherichia coli (strain: APEC O103) 7324510 221218530 pO103_28 YP_002527488.1 Aerobactin siderophore ferric receptor protein IutA Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
3474 iroB from Escherichia coli (strain: APEC O103) Escherichia coli (strain: APEC O103) 7324522 221218540 pO103_42 YP_002527498.1 Salmochelin siderophore glycosyltransferase IroB Virulence factor MUTATION: attenuated in chicks (Caza et al., 2008). 18541653
3475 iroC from Escherichia coli (strain: APEC O103) Escherichia coli (strain: APEC O103) 7324523 221218541 pO103_43 YP_002527499.1 Salmochelin siderophore ATP-binding cassette IroC Virulence factor MUTATION: attenuated in chicks (Caza et al., 2008). 18541653
3476 iroD from Escherichia coli (strain: APEC O103) Escherichia coli (strain: APEC O103) 7324524 221218542 pO103_44 YP_002527500.1 Salmochelin siderophore ferric enterochelin esterase IroD Virulence factor MUTATION: attenuated in chicks (Caza et al., 2008). 18541653
3477 iroN from Escherichia coli (strain: APEC O103) Escherichia coli (strain: APEC O103) 7324526 221218544 pO103_46 YP_002527502.1 Salmochelin siderophore receptor protein IroN Virulence factor MUTATION: iroA gene cluster mutants were unable to resist Lcn2 in mouse serum (Fischbach et al., 2006). 17060628
3478 traT from Escherichia coli (strain: APEC O103) Escherichia coli (strain: APEC O103) 7324607 221218625 pO103_127 YP_002527583.1 conjugal transfer surface exclusion protein TraT Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics (Sukupolvi and O'Connor, 1987). 3323837
3479 traT from Escherichia coli (strain: H30, serovar: O26:H11) Escherichia coli (strain: H30, serovar: O26:H11) 7256873 219586125 pO26I_p078 YP_002456219.1 conjugal transfer surface exclusion protein TraT Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics (Sukupolvi and O'Connor, 1987). 3323837
3480 espF from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7061130 215488961 E2348C_3930 YP_002331392.1 LEE-encoded effector EspF Virulence factor MUTATION: espF mutant of EPEC is deficient in its ability to disrupt electrical resistance across a polarized monolayer of intestinal epithelial cells, suggesting that EspF is an effector molecule required for disrupting intestinal epithelial barrier function (McNamara et al., 2001). 11238563
3481 tonB from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7061491 215486487 E2348C_1379 YP_002328918.1 transport protein TonB Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
3482 rfaH from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7061758 215489181 E2348C_4154 YP_002331612.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice (Nagy et al., 2002). 12117951
3483 map from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7061805 215488973 E2348C_3942 YP_002331404.1 LEE-encoded effector Map Virulence factor MUTATION: map EPEC mutants have significantly reduced levels of invasion of HeLa cells (Jepson et al., 2003). 14531893
3484 fliC from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7061913 215487128 E2348C_2041 YP_002329559.1 flagellin Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains (Erdem et al., 2007). 17693516
3485 fimH from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7062125 215489644 E2348C_4627 YP_002332075.1 minor component of type 1 fimbriae Virulence factor MUTATION: attenuated in mice and humans (Wiles et al., 2008). 18482721
3486 escN from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7062686 215488979 E2348C_3948 YP_002331410.1 translocator EscN Virulence factor MUTATION: deletion of escN abolished pathogenicity in infant rabbits compared to wild-type EHEC (Ritchie and Waldor, 2005). 15731044
3487 ler from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7062691 215488999 E2348C_3968 YP_002331430.1 transcription regulator Ler Virulence factor MUTATION: ler mutants had less adhesion to HeLa cells, ler is a regulator of many virulence genes in the LEE PAI which are needed for adherence (Torres et al., 2008). 18794291
3488 espG from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7062692 215489001 E2348C_3970 YP_002331432.1 LEE-encoded effector EspG Virulence factor MUTATION: the inhibitory effect of the espG mutant on Cl– uptake in Caco-2 monolayers was significantly attenuated as compared with wild-type EPEC (Gill et al., 2007). 17256057
3489 chuA from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7062790 215488778 E2348C_3743 YP_002331209.1 outer membrane heme/hemoglobin receptor Virulence factor MUTATION: chuA mutant unable to grow on iron-depleted medium containing heme (Torres and Payne, 1997). 9157252
3490 ompA from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7063342 215486075 E2348C_0943 YP_002328506.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells (Prasadarao et al., 1999). 10531228
3491 ecpA from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7063831 215485400 E2348C_0249 YP_002327831.1 fimbrillin precursor Virulence factor MUTATION: ecpA mutant had attenuated adherence to HEp-2 cells compared to the parental EHEC strain (Rendón et al., 2007). 17563352
3492 eaeA Escherichia coli O127:H6 str. E2348/69 7063868 215488970 E2348C_3939 YP_002331401.1 intimin EaeA Virulence factor MUTATION: attenuated in humans (Donnenberg et al., 1993). 8376594
3493 espD from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7063878 215488966 E2348C_3935 YP_002331397.1 translocon EspD Virulence factor MUTATION: espD deletion mutant of the enterohemorrhagic E. coli (EHEC) strain EDL933 had impaired attachment to HeLa cells (Kresse et al., 1999). 10456938
3494 espA from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7063880 215488967 E2348C_3936 YP_002331398.1 translocon EspA Virulence factor MUTATION: deletion of espA in STEC abolished ability to attach to HeLa cells and induce actin rearrangements (Ebel et al., 1998). 9786192
3495 dsbA from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7063974 215489194 E2348C_4167 YP_002331625.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC (Donnenberg et al., 1997). 9224871
3496 espB from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7064346 215488965 E2348C_3934 YP_002331396.1 translocon EspB Virulence factor MUTATION: deletion mutant attenuated in humans compared to wild-type EPEC (Tacket et al., 2000). 10816529
3497 espH from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7064347 215488975 E2348C_3944 YP_002331406.1 LEE-encoded effector EspH Virulence factor MUTATION: deletion mutant is attenuated in infant rabbits (Ritchie and Waldor, 2005). 15731044
3498 tir from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7064348 215488972 E2348C_3941 YP_002331403.1 translocated intimin receptor Tir Virulence factor MUTATION: tir deletion strains are unable to adhere to tissue in an in vitro organ culture (Schüller et al., 2007). 17474908
3499 dppA from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7065057 215488827 E2348C_3792 YP_002331258.1 dipeptide transporter Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice (Haugen et al., 2007). 17074858
3500 papG from Escherichia coli UMN026 Escherichia coli UMN026 7155270 218706490 ECUMN_3331 YP_002414009.1 Fimbrial PapG protein (P pilus class II adhesin) (PapGII) Virulence factor MUTATION: attenuated to human neutrophils in vitro (Tewari et al., 1994). 7960108
3501 sat from Escherichia coli UMN026 Escherichia coli UMN026 7155292 218706521 ECUMN_3367 YP_002414040.1 Serine protease precursor (Secreted autotransporter toxin) Virulence factor MUTATION: Sat elicits cytopathic activity that damages host tissue and may increase the ability of E. coli to propagate (Guyer et al., 2002). 12117966
3502 iutA from Escherichia coli UMN026 Escherichia coli UMN026 7155293 218706523 ECUMN_3371 YP_002414042.1 Ferric aerobactin receptor precursor Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
3503 dppA from Escherichia coli UMN026 Escherichia coli UMN026 7155420 218707178 ECUMN_4055 YP_002414697.1 dipeptide transporter ; periplasmic-binding component of ABC superfamily Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice (Haugen et al., 2007). 17074858
3504 aslA from Escherichia coli UMN026 Escherichia coli UMN026 7155811 218707435 ECUMN_4324 YP_002414954.1 arylsulfatase-like enzyme Virulence factor MUTATION: disruption mutant was less invasive in an in vitro HBMEC invasion assay than the wild-type E. coli K1 (Hoffman et al., 2000). 10948126
3505 chuA from Escherichia coli UMN026 Escherichia coli UMN026 7155958 218707130 ECUMN_4000 YP_002414649.1 outer membrane hemin receptor Virulence factor MUTATION: chuA mutant unable to grow on iron-depleted medium containing heme (Torres and Payne, 1997). 9157252
3506 fimH from Escherichia coli UMN026 Escherichia coli UMN026 7156362 218707987 ECUMN_4927 YP_002415506.1 minor component of type 1 fimbriae Virulence factor MUTATION: attenuated in mice in humans (Wiles et al., 2008). 18482721
3507 iucA from Escherichia coli UMN026 Escherichia coli UMN026 7156875 218706527 ECUMN_3375 YP_002414046.1 aerobactin siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3508 iucB from Escherichia coli UMN026 Escherichia coli UMN026 7156876 218706526 ECUMN_3374 YP_002414045.1 aerobactin siderophore biosynthesis protein, N(6)-hydroxylysine acetylase Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3509 iucC from Escherichia coli UMN026 Escherichia coli UMN026 7156877 218706525 ECUMN_3373 YP_002414044.1 Aerobactin siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3510 iucD from Escherichia coli UMN026 Escherichia coli UMN026 7156878 218706524 ECUMN_3372 YP_002414043.1 L-lysine 6-monooxygenase (Lysine N(6)-hydroxylase) Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3511 papA from Escherichia coli UMN026 Escherichia coli UMN026 7157388 218706498 ECUMN_3339 YP_002414017.1 P-fimbriae major subunit precursor Virulence factor MUTATION: mutant papA in wild-type smooth strains do not mediate Gal-Gal adherence (Johnson, 1991). 1672263
3512 rfaH from Escherichia coli UMN026 Escherichia coli UMN026 7158028 218707473 ECUMN_4366 YP_002414992.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice
3513 ecpA from Escherichia coli UMN026 Escherichia coli UMN026 7158311 218703573 ECUMN_0327 YP_002411092.1 E. coli common pilus (ECP) Virulence factor MUTATION: ecpA mutant had attenuated adherence to HEp-2 cells compared to the parental EHEC strain (Rendón et al., 2007). 17563352
3514 ompA from Escherichia coli UMN026 Escherichia coli UMN026 7158500 218704380 ECUMN_1146 YP_002411899.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells (Prasadarao et al., 1999). 10531228
3515 tonB from Escherichia coli UMN026 Escherichia coli UMN026 7158576 218704775 ECUMN_1551 YP_002412294.1 transport protein TonB Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
3516 dsbA from Escherichia coli UMN026 Escherichia coli UMN026 7159290 218707486 ECUMN_4384 YP_002415005.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC (Donnenberg et al., 1997). 9224871
3517 ompA from Escherichia coli IAI39 Escherichia coli IAI39 7149956 218700524 ECIAI39_2190 YP_002408153.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells (Prasadarao et al., 1999). 10531228
3518 tia from Escherichia coli IAI39 Escherichia coli IAI39 7150281 218702439 ECIAI39_4192 YP_002410068.1 Tia invasion determinant Virulence factor MUTATION: tia deletion strains were noninvasive and lacked the ability to adhere to human ileocecal cells (Fleckenstein et al., 1996). 8675335
3519 chuA from Escherichia coli IAI39 Escherichia coli IAI39 7150945 218702265 ECIAI39_4002 YP_002409894.1 outer membrane hemin receptor Virulence factor MUTATION: chuA mutant unable to grow on iron-depleted medium containing heme (Torres and Payne, 1997). 9157252
3520 dppA from Escherichia coli IAI39 Escherichia coli IAI39 7151167 218702310 ECIAI39_4053 YP_002409939.1 dipeptide transporter ; periplasmic-binding component of ABC superfamily Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice (Haugen et al., 2007). 17074858
3521 fimH from Escherichia coli IAI39 Escherichia coli IAI39 7151334 218703019 ECIAI39_4793 YP_002410648.1 minor component of type 1 fimbriae Virulence factor MUTATION: attenuated in mice and humans (Wiles et al., 2008). 18482721
3522 fliC from Escherichia coli IAI39 Escherichia coli IAI39 7151372 218699513 ECIAI39_1132 YP_002407142.1 flagellin Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains (Erdem et al., 2007). 17693516
3523 papA from Escherichia coli IAI39 Escherichia coli IAI39 7151788 0 ECIAI39_3404 - pseudo Virulence factor MUTATION: mutant papA in wild-type smooth strains do not mediate Gal-Gal adherence (Johnson, 1991). 1672263
3524 iucB from Escherichia coli IAI39 Escherichia coli IAI39 7151862 218701707 ECIAI39_3424 YP_002409336.1 aerobactin siderophore biosynthesis protein, N(6)-hydroxylysine acetylase Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3525 iucC from Escherichia coli IAI39 Escherichia coli IAI39 7151863 218701708 ECIAI39_3425 YP_002409337.1 Aerobactin siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3526 iucD from Escherichia coli IAI39 Escherichia coli IAI39 7151864 218701709 ECIAI39_3426 YP_002409338.1 L-lysine 6-monooxygenase (Lysine N(6)-hydroxylase) Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3527 iutA from Escherichia coli IAI39 Escherichia coli IAI39 7151865 218701710 ECIAI39_3427 YP_002409339.1 Ferric aerobactin receptor precursor Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
3528 papA from Escherichia coli IAI39 Escherichia coli IAI39 7152332 218702822 ECIAI39_4587 YP_002410451.1 major pilin protein PapA Virulence factor MUTATION: mutant papA in wild-type smooth strains do not mediate Gal-Gal adherence (Johnson, 1991). 1672263
3529 papG from Escherichia coli IAI39 Escherichia coli IAI39 7152338 218702830 ECIAI39_4595 YP_002410459.1 Fimbrial PapG protein (P pilus class II adhesin) (PapGII) Virulence factor MUTATION: attenuated to human neutrophils in vitro (Tewari et al., 1994). 7960108
3530 tia from Escherichia coli IAI39 Escherichia coli IAI39 7152341 218702831 ECIAI39_4596 YP_002410460.1 Tia invasion determinant Virulence factor MUTATION: tia deletion strains were noninvasive and lacked the ability to adhere to human ileocecal cells (Fleckenstein et al., 1996). 8675335
3531 aslA from Escherichia coli IAI39 Escherichia coli IAI39 7152793 218701288 ECIAI39_2989 YP_002408917.1 arylsulfatase-like enzyme Virulence factor MUTATION: disruption mutant was less invasive in an in vitro HBMEC invasion assay than the wild-type E. coli K1 (Hoffman et al., 2000). 10948126
3532 iucA from Escherichia coli IAI39 Escherichia coli IAI39 7152851 218701706 ECIAI39_3423 YP_002409335.1 aerobactin siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3533 rfaH from Escherichia coli IAI39 Escherichia coli IAI39 7153071 218701455 ECIAI39_3155 YP_002409084.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice (Nagy et al., 2002). 12117951
3534 tonB from Escherichia coli IAI39 Escherichia coli IAI39 7153490 218699959 ECIAI39_1589 YP_002407588.1 transport protein TonB Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
3535 dsbA from Escherichia coli IAI39 Escherichia coli IAI39 7154218 218701440 ECIAI39_3140 YP_002409069.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC (Donnenberg et al., 1997). 9224871
3536 traJ from Escherichia coli UMN026 Escherichia coli UMN026 7144273 218692900 p1ECUMN_0115 YP_002406012.1 co,jugation transfer regulator protein Virulence factor MUTATION: traJ mutants of E. coli K1 are attenuated in rat pups and macrophages (Hill et al., 2004). 14688129
3537 traT from Escherichia coli UMN026 Escherichia coli UMN026 7144350 218692867 p1ECUMN_0081 YP_002405979.1 conjugal transfer surface exclusion protein TraT Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics (Sukupolvi and O'Connor, 1987). 3323837
3538 dppA from Escherichia coli 55989 Escherichia coli 55989 7145054 218697257 EC 55989_3996 YP_002404924.1 dipeptide transporter ; periplasmic-binding component of ABC superfamily Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice (Haugen et al., 2007). 17074858
3539 iutA from Escherichia coli 55989 Escherichia coli 55989 7145114 218697849 EC 55989_4641 YP_002405516.1 Ferric aerobactin receptor precursor Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
3540 iucA from Escherichia coli 55989 Escherichia coli 55989 7146638 218697853 EC 55989_4645 YP_002405520.1 aerobactin siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3541 iucB from Escherichia coli 55989 Escherichia coli 55989 7146639 218697852 EC 55989_4644 YP_002405519.1 aerobactin siderophore biosynthesis protein, N(6)-hydroxylysine acetylase Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3542 iucC from Escherichia coli 55989 Escherichia coli 55989 7146640 218697851 EC 55989_4643 YP_002405518.1 Aerobactin siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3543 iucD from Escherichia coli 55989 Escherichia coli 55989 7146641 218697850 EC 55989_4642 YP_002405517.1 L-lysine 6-monooxygenase (Lysine N(6)-hydroxylase) Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3544 rfaH from Escherichia coli 55989 Escherichia coli 55989 7147756 218697561 EC 55989_4317 YP_002405228.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice (Nagy et al., 2002). 12117951
3545 ecpA from Escherichia coli 55989 Escherichia coli 55989 7148058 218693755 EC 55989_0297 YP_002401422.1 E. coli common pilus (ECP) Virulence factor MUTATION: ecpA mutant had attenuated adherence to HEp-2 cells compared to the parental EHEC strain (Rendón et al., 2007). 17563352
3546 ompA from Escherichia coli 55989 Escherichia coli 55989 7148255 218694432 EC 55989_1006 YP_002402099.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells (Prasadarao et al., 1999). 10531228
3547 tonB from Escherichia coli 55989 Escherichia coli 55989 7148346 218694767 EC 55989_1350 YP_002402434.1 transport protein TonB Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
3548 dsbA from Escherichia coli 55989 Escherichia coli 55989 7149102 218697574 EC 55989_4336 YP_002405241.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC (Donnenberg et al., 1997). 9224871
3549 dppA from Escherichia coli ED1a Escherichia coli ED1a 7140515 218691829 ECED1_4223 YP_002400041.1 dipeptide transporter ; periplasmic-binding component of ABC superfamily Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice (Haugen et al., 2007). 17074858
3550 ecpA from Escherichia coli ED1a Escherichia coli ED1a 7141136 218688179 ECED1_0335 YP_002396391.1 E. coli common pilus (ECP) Virulence factor MUTATION: ecpA mutant had attenuated adherence to HEp-2 cells compared to the parental EHEC strain (Rendón et al., 2007). 17563352
3551 rfaH from Escherichia coli ED1a Escherichia coli ED1a 7141515 218692134 ECED1_4544 YP_002400346.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice (Nagy et al., 2002). 12117951
3552 tonB from Escherichia coli ED1a Escherichia coli ED1a 7141983 218689198 ECED1_1406 YP_002397410.1 transport protein TonB Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
3553 fliC from Escherichia coli ED1a Escherichia coli ED1a 7142129 218689916 ECED1_2188 YP_002398128.1 Flagellin (Phase 1-C flagellin) Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains (Erdem et al., 2007). 17693516
3554 iucA from Escherichia coli ED1a Escherichia coli ED1a 7142472 218692458 ECED1_4905 YP_002400670.1 aerobactin siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3555 iucB from Escherichia coli ED1a Escherichia coli ED1a 7142473 218692457 ECED1_4904 YP_002400669.1 aerobactin siderophore biosynthesis protein, N(6)-hydroxylysine acetylase Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3556 iucC from Escherichia coli ED1a Escherichia coli ED1a 7142474 218692456 ECED1_4903 YP_002400668.1 Aerobactin siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3557 iucD from Escherichia coli ED1a Escherichia coli ED1a 7142475 218692455 ECED1_4902 YP_002400667.1 L-lysine 6-monooxygenase (Lysine N(6)-hydroxylase) Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3558 ompA from Escherichia coli ED1a Escherichia coli ED1a 7142711 218688793 ECED1_0980 YP_002397005.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells (Prasadarao et al., 1999). 10531228
3559 dsbA from Escherichia coli ED1a Escherichia coli ED1a 7142882 218692147 ECED1_4561 YP_002400359.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC (Donnenberg et al., 1997). 9224871
3560 iutA from Escherichia coli ED1a Escherichia coli ED1a 7143612 218692454 ECED1_4901 YP_002400666.1 Ferric aerobactin receptor precursor Virulence factor MUTATION: attenuated in mice. (Torres et al., 2001). 11553558
3561 iutA from Escherichia coli S88 Escherichia coli S88 7119366 218534486 pECS88_0009 YP_002401016.1 Ferric aerobactin receptor precusor IutA Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
3562 iroN from Escherichia coli S88 Escherichia coli S88 7119392 218534588 pECS88_0124 YP_002401118.1 outer membrane receptor FepA Virulence factor MUTATION: iroA gene cluster mutants were unable to resist Lcn2 in mouse serum (Fischbach et al., 2006). 17060628
3563 iroB from Escherichia coli S88 Escherichia coli S88 7119430 218534592 pECS88_0128 YP_002401122.1 IroB, putative glucosyltransferase Virulence factor MUTATION: attenuated in chicks (Caza et al., 2008). 18541653
3564 iroC from Escherichia coli S88 Escherichia coli S88 7119431 218534591 pECS88_0127 YP_002401121.1 IroC, ATP binding cassette (ABC) transporter homolog Virulence factor MUTATION: attenuated in chicks (Caza et al., 2008). 18541653
3565 iroD from Escherichia coli S88 Escherichia coli S88 7119432 218534590 pECS88_0126 YP_002401120.1 IroD, putative ferric enterochelin esterase Virulence factor MUTATION: attenuated in chicks (Caza et al., 2008). 18541653
3566 iucA from Escherichia coli S88 Escherichia coli S88 7119436 218534490 pECS88_0013 YP_002401020.1 Aerobactin siderophore biosynthesis protein IucA Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3567 iucB from Escherichia coli S88 Escherichia coli S88 7119437 218534489 pECS88_0012 YP_002401019.1 N(6)-hydroxylysine acetylase IucB Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3568 iucC from Escherichia coli S88 Escherichia coli S88 7119438 218534488 pECS88_0011 YP_002401018.1 Aerobactin siderophore biosynthesis protein IucC Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3569 iucD from Escherichia coli S88 Escherichia coli S88 7119439 218534487 pECS88_0010 YP_002401017.1 L-lysine 6-monooxigenase IucD Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3570 traJ from Escherichia coli S88 Escherichia coli S88 7119465 218534534 pECS88_0064 YP_002401064.1 conjugal transfer transcriptional regulator TraJ Virulence factor MUTATION: traJ mutants of E. coli K1 are attenuated in rat pups and macrophages (Hill et al., 2004). 14688129
3571 traT from Escherichia coli S88 Escherichia coli S88 7119470 218534560 pECS88_0092 YP_002401090.1 conjugal transfer surface exclusion protein TraT Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics (Sukupolvi and O'Connor, 1987). 3323837
3572 fimH from Escherichia coli S88 Escherichia coli S88 7129499 218561496 ECS88_4938 YP_002394409.1 minor component of type 1 fimbriae Virulence factor MUTATION: attenuated in mice and humans (Wiles et al., 2008). 18482721
3573 aslA from Escherichia coli S88 Escherichia coli S88 7129944 218560864 ECS88_4222 YP_002393777.1 arylsulfatase-like enzyme Virulence factor MUTATION: disruption mutant was less invasive in an in vitro HBMEC invasion assay than the wild-type E. coli K1 (Hoffman et al., 2000). 10948126
3574 ompA from Escherichia coli S88 Escherichia coli S88 7130155 218557855 ECS88_0978 YP_002390768.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells (Prasadarao et al., 1999). 10531228
3575 dppA from Escherichia coli S88 Escherichia coli S88 7130233 218560622 ECS88_3964 YP_002393535.1 dipeptide transporter ; periplasmic-binding component of ABC superfamily Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice (Haugen et al., 2007). 17074858
3576 ecpA from Escherichia coli S88 Escherichia coli S88 7130299 218557210 ECS88_0290 YP_002390123.1 E. coli common pilus (ECP) Virulence factor MUTATION: ecpA mutant had attenuated adherence to HEp-2 cells compared to the parental EHEC strain (Rendón et al., 2007). 17563352
3577 dsbA from Escherichia coli S88 Escherichia coli S88 7130870 218560936 ECS88_4310 YP_002393849.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC (Donnenberg et al., 1997). 9224871
3578 rfaH from Escherichia coli S88 Escherichia coli S88 7131265 218560923 ECS88_4290 YP_002393836.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice (Nagy et al., 2002). 12117951
3579 tonB from Escherichia coli S88 Escherichia coli S88 7131480 218558181 ECS88_1322 YP_002391094.1 transport protein TonB Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
3580 chuA from Escherichia coli S88 Escherichia coli S88 7131907 218560576 ECS88_3913 YP_002393489.1 outer membrane hemin receptor Virulence factor MUTATION: chuA mutant unable to grow on iron-depleted medium containing heme (Torres and Payne, 1997). 9157252
3581 FliC from Escherichia coli S88 Escherichia coli S88 7132788 218558781 ECS88_1977 YP_002391694.1 flagellin Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains (Erdem et al., 2007). 17693516
3582 papA from Escherichia coli S88 Escherichia coli S88 7133169 218559971 ECS88_3265 YP_002392884.1 major pilin protein PapA Virulence factor MUTATION: mutant papA in wild-type smooth strains do not mediate Gal-Gal adherence (Johnson, 1991). 1672263
3583 tia from Escherichia coli S88 Escherichia coli S88 7133342 218559962 ECS88_3256 YP_002392875.1 Tia invasion determinant Virulence factor MUTATION: tia deletion strains were noninvasive and lacked the ability to adhere to human ileocecal cells (Fleckenstein et al., 1996). 8675335
3584 papG from Escherichia coli S88 Escherichia coli S88 7133471 218559963 ECS88_3257 YP_002392876.1 Fimbrial PapG protein (P pilus class II adhesin) (PapGII) Virulence factor MUTATION: attenuated to human neutrophils in vitro (Tewari et al., 1994). 7960108
3585 ompA from Escherichia coli IAI1 Escherichia coli IAI1 7124740 218553544 ECIAI1_0998 YP_002386457.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells (Prasadarao et al., 1999). 10531228
3586 dppA from Escherichia coli IAI1 Escherichia coli IAI1 7125629 218556099 ECIAI1_3703 YP_002389012.1 dipeptide transporter ; periplasmic-binding component of ABC superfamily Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice (Haugen et al., 2007). 17074858
3587 rfaH from Escherichia coli IAI1 Escherichia coli IAI1 7126217 218556406 ECIAI1_4035 YP_002389320.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice (Nagy et al., 2002). 12117951
3588 ecpA from Escherichia coli IAI1 Escherichia coli IAI1 7126700 218552859 ECIAI1_0294 YP_002385772.1 E. coli common pilus (ECP) Virulence factor MUTATION: ecpA mutant had attenuated adherence to HEp-2 cells compared to the parental EHEC strain (Rendón et al., 2007). 17563352
3589 fliC from Escherichia coli IAI1 Escherichia coli IAI1 7126825 218554508 ECIAI1_2009 YP_002387421.1 flagellin Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains (Erdem et al., 2007). 17693516
3590 fimH from Escherichia coli IAI1 Escherichia coli IAI1 7127116 218556851 ECIAI1_4536 YP_002389765.1 minor component of type 1 fimbriae Virulence factor MUTATION: attenuated in mice and humans (Wiles et al., 2008). 18482721
3591 tonB from Escherichia coli IAI1 Escherichia coli IAI1 7127821 218553804 ECIAI1_1272 YP_002386717.1 transport protein TonB Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
3592 dsbA from Escherichia coli IAI1 Escherichia coli IAI1 7128194 218556419 ECIAI1_4059 YP_002389333.1 periplasmic protein disulfide isomerase I Virulence factor, Protective antigen MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC (Donnenberg et al., 1997). 9224871
3593 bfpE from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7056322 215276200 E2348_P1_009 YP_002332163.1 transmembrane protein Virulence factor MUTATION: bfpE mutant strain of EPEC fails to form detectable BFP and fails to carry out autoaggregation and localized adherence to HEp-2 epithelial cells in tissue culture (Blank and Donnenberg, 2001). 11443077
3594 bfpF from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7056323 215276201 E2348_P1_010 YP_002332164.1 nucleotide binding protein Virulence factor MUTATION: bfpF mutant colonized the human intestine but was about 200-fold less virulent than the wild-type EPEC (Bieber et al., 1998). 9641917
3595 bfpP from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7056324 215276202 E2348_P1_011 YP_002332165.1 prepilin peptidase Virulence factor MUTATION: mutation in bfpP blocks BFP biogenesis and localized adherence to host cells by EPEC (Anantha et al., 2000). 10762251
3596 bfpB from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7056325 215276196 E2348_P1_005 YP_002332159.1 lipoprotein Virulence factor MUTATION: bfpB mutant EPEC is unable to form BFP which is a major virulence factor of EPEC (Ramer et al., 1996). 8932312
3597 bfpA from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7056343 215276194 E2348_P1_003 YP_002332157.1 major pilin structural unit bundlin Virulence factor MUTATION: bfpA deletion mutant of EPEC was less virulent in humans than the wild-type EPEC (Bieber et al., 1998). 9641917
3598 traT from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7056350 215276226 E2348_P1_046 YP_002332189.1 conjugal transfer surface exclusion protein TraT Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics (Sukupolvi and O'Connor, 1987). 3323837
3599 traJ from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7056370 215276255 E2348_P1_075 YP_002332218.1 transcriptional activator Virulence factor MUTATION: traJ mutants of E. coli K1 are attenuated in rat pups and macrophages (Hill et al., 2004). 14688129
3678 toxB from Escherichia coli O26:H- Escherichia coli O26:H- 8715786 284000161 pO26CRL_0032 YP_003377848.1 toxin B Virulence factor MUTATION: toxB mutants had attenuated adherence to Caco-2 cells (Tatsuno et al., 2001). 11598035
3679 hlyC from Escherichia coli O26:H- Escherichia coli O26:H- 8715862 284000229 pO26CRL_0108 YP_003377916.1 hemolysin C Virulence factor MUTATION: hlyC is required for the acylation and therefore activation of the toxin hemolysin which has the ability to lyse several different types of cells (Stanley et al., 1996). 8793877
3680 hlyA from Escherichia coli O26:H- Escherichia coli O26:H- 8715863 284000230 pO26CRL_0109 YP_003377917.1 hemolysin A Virulence factor MUTATION: attenuated in zebra fish (Wiles et al., 2009). 20019794
3681 ompA from Escherichia coli B str. REL606 Escherichia coli B str. REL606 8175755 254161071 ECB_00961 YP_003044179.1 outer membrane protein A (3a;II*;G;d) Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells (Prasadarao et al., 1999). 10531228
3682 dppA from Escherichia coli B str. REL606 Escherichia coli B str. REL606 8177616 254163468 ECB_03395 YP_003046576.1 dipeptide transporter Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice (Haugen et al., 2007). 17074858
3683 rfaH from Escherichia coli B str. REL606 Escherichia coli B str. REL606 8177876 254163799 ECB_03733 YP_003046907.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice (Nagy et al., 2002). 12117951
3684 dsbA from Escherichia coli B str. REL606 Escherichia coli B str. REL606 8177889 254163812 ECB_03746 YP_003046920.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC (Donnenberg et al., 1997). 9224871
3685 fimH from Escherichia coli B str. REL606 Escherichia coli B str. REL606 8178231 254164241 ECB_04189 YP_003047351.1 minor component of type 1 fimbriae Virulence factor MUTATION: attenuated in mice and humans (Wiles et al., 2008). 18482721
3686 tonB from Escherichia coli B str. REL606 Escherichia coli B str. REL606 8178456 254161331 ECB_01226 YP_003044439.1 membrane spanning protein in TonB-ExbB-ExbD complex Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
3687 traT from Escherichia coli (strain: EC 14, pathovar: UPEC, nat-host: Homo sapiens) Escherichia coli (strain: EC 14, pathovar: UPEC, nat-host: Homo sapiens) 8381950 256855359 pEC 14_110 YP_003162603.1 IncF plasmid conjugative transfer surface exclusion protein TraT Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics (Sukupolvi and O'Connor, 1987). 3323837
3688 tir from Escherichia coli (strain: A, other: UK epidemic CTX-M-producing strain (PFGE-defined); MLST-defined ST131 linea Escherichia coli (strain: A, other: UK epidemic CTX-M-producing strain (PFGE-defined); MLST-defined 8319255 256367770 pEK499_p062 YP_003108327.1 inner membrane protein Virulence factor MUTATION: tir deletion strains are unable to adhere to tissue in an in vitro organ culture (Schüller et al., 2007). 17474908
3689 tir from Escherichia coli (strain: D, other: UK epidemic CTX-M-producing strain (PFGE-defined); MLST-defined ST131 linea Escherichia coli (strain: D, other: UK epidemic CTX-M-producing strain (PFGE-defined); MLST-defined 8319157 256367694 pEK516_p77 YP_003108263.1 Tir Virulence factor MUTATION: tir deletion strains are unable to adhere to tissue in an in vitro organ culture (Schüller et al., 2007). 17474908
3690 eltA from Escherichia coli ETEC H10407 Escherichia coli ETEC H10407 8571459 268507198 pEntH10407_p39 YP_003293997.1 heat-labile enterotoxin A subunit Virulence factor MUTATION: Deletion mutants of eltA and eltB had decreased levels of inducing cAMP secretion in an in vitro culture system, and had less adherence to IPEC-J2 and Caco-2 cells than the wild-type ETEC (Johnson et al., 2009). 18978047
3691 eltB Escherichia coli ETEC H10407 8571464 268507197 pEntH10407_p38 AP010910 YP_003293996 heat-labile enterotoxin B subunit Virulence factor MUTATION: Deletion mutants of eltA and eltB had decreased levels of inducing cAMP secretion in an in vitro culture system, and had less adherence to IPEC-J2 and Caco-2 cells than the wild-type ETEC (Johnson et al., 2009). 18978047
3692 hcpA Escherichia coli O157:H7 str. TW14359 8214675 254791243 ECSP_0109 CP001368 YP_003076080 putative major pilin subunit Virulence factor MUTATION: hcpA deletion mutant had attenuated invasion of cultured epithelial cells compared to wild-type EHEC (Xicohtencatl-Cortes et al., 2009). 18952791
3693 ecpA from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8214896 254791452 ECSP_0330 YP_003076289.1 Mat fimbrillin Virulence factor MUTATION: ecpA mutant had attenuated adherence to HEp-2 cells compared to the parental EHEC strain (Rendón et al., 2007). 17563352
3694 ompA from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8215629 254792157 ECSP_1063 YP_003076994.1 outer membrane protein A (3a;II*;G;d) Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells (Prasadarao et al., 1999). 10531228
3695 espK Escherichia coli O157:H7 str. TW14359 8216050 254792564 ECSP_1484 CP001368 YP_003077401 non-LEE-encoded type III secreted effector Virulence factor MUTATION: espK mutant attenuated in calves (Vlisidou et al., 2006). 16958848
3696 tonB from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8216210 254792722 ECSP_1644 YP_003077559.1 TonB protein Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
3697 nleA Escherichia coli O157:H7 str. TW14359 8216268 254792777 ECSP_1702 CP001368 YP_003077614 non-LEE-encoded type III secreted effector Virulence factor MUTATION: nelA deletion mutant attenuated in mice (Gruenheid et al., 2004). 14982621
3698 fliC from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8217094 254793574 ECSP_2528 YP_003078411.1 flagellar filament structural protein (flagellin) Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains (Erdem et al., 2007). 17693516
3699 stx2B from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8217818 254794270 ECSP_3252 CP001368 YP_003079107 shiga toxin II subunit B Virulence factor TOXIN: Stx induces apoptosis in HeLa cells (Cherla et al., 2003). 14638419
3700 chuA from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219055 254795452 ECSP_4489 YP_003080289.1 outer membrane heme/hemoglobin receptor Virulence factor MUTATION: chuA mutant unable to grow on iron-depleted medium containing heme (Torres and Payne, 1997). 9157252
3701 dppA from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219101 254795498 ECSP_4535 YP_003080335.1 dipeptide transporter, periplasmic-binding component of ABC superfamily Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice (Haugen et al., 2007). 17074858
3702 espF from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219231 254795625 ECSP_4665 YP_003080462.1 LEE-encoded type III secreted effector Virulence factor MUTATION: espF mutant of EPEC is deficient in its ability to disrupt electrical resistance across a polarized monolayer of intestinal epithelial cells, suggesting that EspF is an effector molecule required for disrupting intestinal epithelial barrier function (McNamara et al., 2001). 11238563
3703 espB from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219235 254795629 ECSP_4669 YP_003080466.1 secreted protein EspB Virulence factor MUTATION: deletion mutant attenuated in humans compared to wild-type EPEC (Tacket et al., 2000). 10816529
3704 espD from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219236 254795630 ECSP_4670 YP_003080467.1 secreted protein EspD Virulence factor MUTATION: espD deletion mutant of the enterohemorrhagic E. coli (EHEC) strain EDL933 had impaired attachment to HeLa cells (Kresse et al., 1999). 10456938
3705 espA from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219237 254795631 ECSP_4671 YP_003080468.1 secreted protein EspA Virulence factor MUTATION: deletion of espA in STEC abolished ability to attach to HeLa cells and induce actin rearrangements (Ebel et al., 1998). 9786192
3706 eae from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219240 254795634 ECSP_4674 YP_003080471.1 intimin adherence protein Virulence factor MUTATION: attenuated in humans (Donnenberg et al., 1993). 8376594
3707 tir from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219242 254795636 ECSP_4676 YP_003080473.1 translocated intimin receptor protein Virulence factor MUTATION: tir deletion strains are unable to adhere to tissue in an in vitro organ culture (Schüller et al., 2007). 17474908
3708 espH from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219245 254795639 ECSP_4679 YP_003080476.1 LEE-encoded type III secreted effector Virulence factor MUTATION: deletion mutant is attenuated in infant rabbits (Ritchie and Waldor, 2005). 15731044
3709 escN from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219249 254795643 ECSP_4683 YP_003080480.1 LEE-encoded type III secretion system factor Virulence factor MUTATION: deletion of escN abolished pathogenicity in infant rabbits compared to wild-type EHEC (Ritchie and Waldor, 2005). 15731044
3710 ler from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219269 254795663 ECSP_4703 YP_003080500.1 locus of enterocyte effacement (LEE)-encoded regulator Virulence factor MUTATION: ler mutants had less adhesion to HeLa cells, ler is a regulator of many virulence genes in the LEE PAI which are needed for adherence (Torres et al., 2008). 18794291
3711 espG from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219270 254795664 ECSP_4704 YP_003080501.1 LEE-encoded type III secreted effector Virulence factor MUTATION: the inhibitory effect of the espG mutant on Cl– uptake in Caco-2 monolayers was significantly attenuated as compared with wild-type EPEC (Gill et al., 2007). 17256057
3712 rfaH from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219461 254795838 ECSP_4895 YP_003080675.1 DNA-binding transcriptional antiterminator Virulence factor MUTATION: attenuated in mice (Nagy et al., 2002). 12117951
3713 dsbA from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219479 254795851 ECSP_4913 YP_003080688.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC (Donnenberg et al., 1997). 9224871
3714 fimH from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219970 254796322 ECSP_5404 YP_003081159.1 minor fimbrial subunit, D-mannose specific adhesin Virulence factor MUTATION: attenuated in mice and humans (Wiles et al., 2008). 18482721
3715 iucA from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8484733 260867380 ECO111_1293 YP_003233782.1 siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3716 espG from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8485562 260869665 ECO111_3731 YP_003236067.1 T3SS secreted effector EspG Virulence factor MUTATION: the inhibitory effect of the espG mutant on Cl– uptake in Caco-2 monolayers was significantly attenuated in as compared with wild-type EPEC
3717 espF from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8485564 260869668 ECO111_3734 YP_003236070.1 T3SS secreted effector EspF Virulence factor MUTATION: espF mutant of EPEC is deficient in its ability to disrupt electrical resistance across a polarized monolayer of intestinal epithelial cells, suggesting that EspF is an effector molecule required for disrupting intestinal epithelial barrier function (McNamara et al., 2001). 11238563
3718 escN from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8485567 260869686 ECO111_3752 YP_003236088.1 translocator EscN Virulence factor MUTATION: deletion of escN abolished pathogenicity in infant rabbits compared to wild-type EHEC (Ritchie and Waldor, 2005). 15731044
3719 ler from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8485572 260869706 ECO111_3772 YP_003236108.1 transcription regulator Ler Virulence factor MUTATION: ler mutants had less adhesion to HeLa cells, ler is a regulator of many virulence genes in the LEE PAI which are needed for adherence (Torres et al., 2008). 18794291
3720 efa1/lifA from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8485768 260870884 ECO111_5002 YP_003237286.1 Efa1/LifA-like protein Virulence factor MUTATION: deletion mutant lacks the ability to inhibit lymphokine production and lymphocyte proliferation compared to wild type EPEC (Klapproth et al., 2000). 10722613
3721 espB from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8486116 260869672 ECO111_3738 YP_003236074.1 translocon EspB Virulence factor MUTATION: deletion mutant attenuated in humans compared to wild-type EPEC (Tacket et al., 2000). 10816529
3722 espH from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8486117 260869682 ECO111_3748 YP_003236084.1 T3SS secreted effector EspH Virulence factor MUTATION: deletion mutant is attenuated in infant rabbits (Ritchie and Waldor, 2005). 15731044
3723 tir from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8486118 260869679 ECO111_3745 YP_003236081.1 putative translocated intimin receptor Tir Virulence factor MUTATION: tir deletion strains are unable to adhere to tissue in an in vitro organ culture (Schüller et al., 2007). 17474908
3724 dppA from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8486355 260870271 ECO111_4360 YP_003236673.1 dipeptide transport protein Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice (Haugen et al., 2007). 17074858
3725 eae from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8486421 260869677 ECO111_3743 YP_003236079.1 theta intimin Virulence factor MUTATION: attenuated in humans (Donnenberg et al., 1993). 8376594
3726 espD from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8486431 260869673 ECO111_3739 YP_003236075.1 translocon EspD Virulence factor MUTATION: espD deletion mutant of the enterohemorrhagic E. coli (EHEC) strain EDL933 had impaired attachment to HeLa cells (Kresse et al., 1999). 10456938
3727 espA from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8486432 260869674 ECO111_3740 YP_003236076.1 translocon EspA Virulence factor MUTATION: deletion of espA in STEC abolished ability to attach to HeLa cells and induce actin rearrangements (Ebel et al., 1998). 9786192
3728 fimH from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8486516 260871042 ECO111_5176 YP_003237444.1 minor component FimH of type 1 fimbriae Virulence factor MUTATION: attenuated in mice and humans (Wiles et al., 2008). 18482721
3729 fliC from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8486550 260868515 ECO111_2503 YP_003234917.1 flagellin Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains (Erdem et al., 2007). 17693516
3730 iucB from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8487016 260867381 ECO111_1294 YP_003233783.1 siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3731 iucC from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8487017 260867382 ECO111_1295 YP_003233784.1 siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3732 iucD from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8487018 260867383 ECO111_1296 YP_003233785.1 siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3733 iutA from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8487019 260867384 ECO111_1297 YP_003233786.1 putative ferric siderophore receptor Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
3734 rfaH from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8488159 260870564 ECO111_4668 YP_003236966.1 DNA-binding transcriptional antiterminator RfaH Virulence factor MUTATION: attenuated in mice (Nagy et al., 2002). 12117951
3735 map from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8488207 260869680 ECO111_3746 YP_003236082.1 T3SS secreted effector Map Virulence factor MUTATION: map EPEC mutants have significantly reduced levels of invasion of HeLa cells (Jepson et al., 2003). 14531893
3736 ompA from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8488643 260867129 ECO111_1025 YP_003233531.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells (Prasadarao et al., 1999). 10531228
3737 tonB from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8488732 260868022 ECO111_1986 YP_003234424.1 membrane spanning protein TonB Virulence factor MUTATION: attenuated in mice (Torres et al., 2001). 11553558
3738 dsbA from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8489430 260870577 ECO111_4681 YP_003236979.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC (Donnenberg et al., 1997). 9224871
3739 iucA from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8479008 260854496 ECO26_1345 YP_003228387.1 siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice (de and Martinez, 1988). 2973985
3740 espG from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8480236 260858241 ECO26_5250 YP_003232132.1 T3SS secreted effector EspG Virulence factor MUTATION: the inhibitory effect of the espG mutant on Cl– uptake in Caco-2 monolayers was significantly attenuated as compared with wild-type EPEC (Gill et al., 2007). 17256057
3741 espF from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8480244 260858280 ECO26_5289 YP_003232171.1 T3SS secreted effector EspF Virulence factor MUTATION: espF mutant of EPEC is deficient in its ability to disrupt electrical resistance across a polarized monolayer of intestinal epithelial cells, suggesting that EspF is an effector molecule required for disrupting intestinal epithelial barrier function (McNamara et al., 2001). 11238563
3742 efa1/lifA fromEscherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8480245 260858281 ECO26_5291 YP_003232172.1 Efa1/LifA-like protein Virulence factor MUTATION: deletion mutant lacks the ability to inhibit lymphokine production and lymphocyte proliferation compared to wild type EPEC (Klapproth et al., 2000). 10722613
3743 map from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8480654 260858268 ECO26_5277 YP_003232159.1 T3SS secreted effector Map Virulence factor MUTATION: map EPEC mutants have significantly reduced levels of invasion of HeLa cells (Jepson et al., 2003). 14531893
3744 eae from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8480655 260858271 ECO26_5280 YP_003232162.1 intimin Virulence factor MUTATION: attenuated in humans (Donnenberg et al., 1993). 8376594
3745 dppA from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8480894 260857652 ECO26_4638 YP_003231543.1 dipeptide transport protein Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice (Haugen et al., 2007). 17074858
3746 matB/ecpA from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8480920 260853523 ECO26_0328 YP_003227414.1 putative fimbrillin precursor Virulence factor MUTATION: ecpA mutant had attenuated adherence to HEp-2 cells compared to the parental EHEC strain (Rendón et al., 2007). 17563352